Extracorporeal bioartificial liver using the radial-flow bioreactor in treatment of fatal experimental hepatic encephalopathy

Hideki Kanai; Hideki Marushima; Naofumi Kimura; Takamasa Iwaki; Masaya Saito; Haruka Maehashi; Keiko Shimizu; Makiko Muto; Takahiro Masaki; Kiyoshi Ohkawa; Keitaro Yokoyama; Masaaki Nakayama; Tohru Harada; Hiroshi Hano; Yoshiaki Hataba; Takahiro Fukuda; Masahiko Nakamura; Naoto Totsuka; Shutaro Ishikawa; Yasuki Unemura; Yuji Ishii; Katsuhiko Yanaga; Tomokazu Matsuura

doi:10.1111/j.1525-1594.2007.00354.x

Extracorporeal bioartificial liver using the radial-flow bioreactor in treatment of fatal experimental hepatic encephalopathy

Hideki Kanai, Hideki Marushima, Naofumi Kimura, Takamasa Iwaki, Masaya Saito, Haruka Maehashi, Keiko Shimizu, Makiko Muto, Takahiro Masaki, Kiyoshi Ohkawa, Keitaro Yokoyama, Masaaki Nakayama, Tohru Harada, Hiroshi Hano, Yoshiaki Hataba, Takahiro Fukuda, Masahiko Nakamura, Naoto Totsuka, Shutaro Ishikawa, Yasuki UnemuraYuji Ishii, Katsuhiko Yanaga, Tomokazu Matsuura

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

An extracorporeal bioartificial liver (BAL) that could prevent death from hepatic encephalopathy in acute hepatic insufficiency was aimed to develop. A functional human hepatocellular carcinoma cell line (FLC-4) was cultured in a radial-flow bioreactor. The function of the BAL was tested in mini-pigs with acute hepatic failure induced by α-amanitin and lipopolysaccharide. When the BAL system was connected with cultured FLC-4 to three pigs with hepatic dysfunction, all demonstrated electroencephalographic improvement and survived. Relatively low plasma concentrations of S-100 β protein, as a marker of astrocytic damage, from pigs with hepatic failure during BAL therapy were noted. BAL therapy can prevent irreversible brain damage from hepatic encephalopathy in experimental acute hepatic failure.

Original language	English
Pages (from-to)	148-151
Number of pages	4
Journal	Artificial Organs
Volume	31
Issue number	2
DOIs	https://doi.org/10.1111/j.1525-1594.2007.00354.x
Publication status	Published - 2007 Feb
Externally published	Yes

Keywords

Acute hepatic failure
Astrocytes
Cerebral edema
Radial-flow bioreactor
α-Amanitin

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomaterials
Biomedical Engineering

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10.1111/j.1525-1594.2007.00354.x

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Kanai, H., Marushima, H., Kimura, N., Iwaki, T., Saito, M., Maehashi, H., Shimizu, K., Muto, M., Masaki, T., Ohkawa, K., Yokoyama, K., Nakayama, M., Harada, T., Hano, H., Hataba, Y., Fukuda, T., Nakamura, M., Totsuka, N., Ishikawa, S., ... Matsuura, T. (2007). Extracorporeal bioartificial liver using the radial-flow bioreactor in treatment of fatal experimental hepatic encephalopathy. Artificial Organs, 31(2), 148-151. https://doi.org/10.1111/j.1525-1594.2007.00354.x

Kanai, H, Marushima, H, Kimura, N, Iwaki, T, Saito, M, Maehashi, H, Shimizu, K, Muto, M, Masaki, T, Ohkawa, K, Yokoyama, K, Nakayama, M, Harada, T, Hano, H, Hataba, Y, Fukuda, T, Nakamura, M, Totsuka, N, Ishikawa, S, Unemura, Y, Ishii, Y, Yanaga, K & Matsuura, T 2007, 'Extracorporeal bioartificial liver using the radial-flow bioreactor in treatment of fatal experimental hepatic encephalopathy', Artificial Organs, vol. 31, no. 2, pp. 148-151. https://doi.org/10.1111/j.1525-1594.2007.00354.x

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abstract = "An extracorporeal bioartificial liver (BAL) that could prevent death from hepatic encephalopathy in acute hepatic insufficiency was aimed to develop. A functional human hepatocellular carcinoma cell line (FLC-4) was cultured in a radial-flow bioreactor. The function of the BAL was tested in mini-pigs with acute hepatic failure induced by α-amanitin and lipopolysaccharide. When the BAL system was connected with cultured FLC-4 to three pigs with hepatic dysfunction, all demonstrated electroencephalographic improvement and survived. Relatively low plasma concentrations of S-100 β protein, as a marker of astrocytic damage, from pigs with hepatic failure during BAL therapy were noted. BAL therapy can prevent irreversible brain damage from hepatic encephalopathy in experimental acute hepatic failure.",

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author = "Hideki Kanai and Hideki Marushima and Naofumi Kimura and Takamasa Iwaki and Masaya Saito and Haruka Maehashi and Keiko Shimizu and Makiko Muto and Takahiro Masaki and Kiyoshi Ohkawa and Keitaro Yokoyama and Masaaki Nakayama and Tohru Harada and Hiroshi Hano and Yoshiaki Hataba and Takahiro Fukuda and Masahiko Nakamura and Naoto Totsuka and Shutaro Ishikawa and Yasuki Unemura and Yuji Ishii and Katsuhiko Yanaga and Tomokazu Matsuura",

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AU - Saito, Masaya

AU - Maehashi, Haruka

AU - Shimizu, Keiko

AU - Muto, Makiko

AU - Masaki, Takahiro

AU - Ohkawa, Kiyoshi

AU - Yokoyama, Keitaro

AU - Nakayama, Masaaki

AU - Harada, Tohru

AU - Hano, Hiroshi

AU - Hataba, Yoshiaki

AU - Fukuda, Takahiro

AU - Nakamura, Masahiko

AU - Totsuka, Naoto

AU - Ishikawa, Shutaro

AU - Unemura, Yasuki

AU - Ishii, Yuji

AU - Yanaga, Katsuhiko

AU - Matsuura, Tomokazu

PY - 2007/2

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KW - Cerebral edema

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Extracorporeal bioartificial liver using the radial-flow bioreactor in treatment of fatal experimental hepatic encephalopathy

Abstract

Keywords

ASJC Scopus subject areas

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