Abstract
An extracorporeal bioartificial liver (BAL) that could prevent death from hepatic encephalopathy in acute hepatic insufficiency was aimed to develop. A functional human hepatocellular carcinoma cell line (FLC-4) was cultured in a radial-flow bioreactor. The function of the BAL was tested in mini-pigs with acute hepatic failure induced by α-amanitin and lipopolysaccharide. When the BAL system was connected with cultured FLC-4 to three pigs with hepatic dysfunction, all demonstrated electroencephalographic improvement and survived. Relatively low plasma concentrations of S-100 β protein, as a marker of astrocytic damage, from pigs with hepatic failure during BAL therapy were noted. BAL therapy can prevent irreversible brain damage from hepatic encephalopathy in experimental acute hepatic failure.
Original language | English |
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Pages (from-to) | 148-151 |
Number of pages | 4 |
Journal | Artificial Organs |
Volume | 31 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2007 Feb |
Externally published | Yes |
Keywords
- Acute hepatic failure
- Astrocytes
- Cerebral edema
- Radial-flow bioreactor
- α-Amanitin
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomaterials
- Biomedical Engineering