Extracellular ATP potentiates steroidogenic effect of adrenocorticotropic hormone in bovine adrenocortical fasciculata cells

M. Kawamura, A. Niitsu, H. Nishi, E. Masaki

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9 Citations (Scopus)

Abstract

We examined the effect of extracellular adenosine 5′-triphosphate (ATP) on adrenocorticotropic hormone (ACTH)- and angiotensin II-induced steroidogenesis in bovine adrenocortical fasciculata cells. The low concentration of ATP (5 μM) potentiated ACTH-induced steroidogenesis synergistically. However, the purine derivative did not affect angiotensin II-induced steroidogenesis. Although adenosine (100 μM) (a metabolite of ATP) showed a weak steroidogenic effect, it did not potentiate ACTH-induced steroidogenesis. ATp also enhanced the steroidogenesis by NaF synergistically in bovine adrenocortical cells, but did not potentiate forskolin- and dibutyryl cyclic AMP-induced steroidogenesis. The stimulating effect of ACTH on cyclic AMP production was synergistically accelerated by ATP (5 μM), which has no effect by itself on cyclic AMP formation. These results suggest that extracellular ATP affected the ACTH receptor-adenylyl cyclase coupling processes, and potentiation of steroidogenesis by ACTH ensued in bovine adrenocortical fasciculata cells.

Original languageEnglish
Pages (from-to)376-381
Number of pages6
JournalJapanese Journal of Pharmacology
Volume85
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • ATP
  • Adrenal cortex
  • Adrenocorticotropic hormone
  • P2Y receptor
  • Steroidogenesis

ASJC Scopus subject areas

  • Pharmacology

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