Extended proliferation of chicken- and Okinawa rail-derived fibroblasts by expression of cell cycle regulators

Masafumi Katayama, Tohru Kiyono, Hitomi Ohmaki, Takahiro Eitsuka, Daiji Endoh, Miho Inoue-Murayama, Nobuyoshi Nakajima, Manabu Onuma, Tomokazu Fukuda

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Although immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian-derived cells. We introduced the simian virus 40 T antigen (SV40T) and human papillomavirus (HPV)-E6E7 to chick and Okinawa rail (endangered species)derived fibroblast. As a result, neither the SV40T nor E6E7 genes could induce avian cell immortality. Accordingly, we attempted to use a recently developed immortalization method, which involved the coexpression of mutant cyclin-dependent kinase 4 (CDK4), Cyclin D, and TERT (K4DT method) in these avian cells. Although the K4DT method could not efficiently induce the efficient immortalization in mass cell population, cellular division until the senescence was significantly extended by K4DT, we succeeded to obtain the immortalized avian cells (chick K4DT: one clone, Okinawa rail K4DT: three clones, Okinawa rail K4DT + telomerase RNA component: one clone) with K4DT expression. We conclude that K4DT expression is used to extend the cell division and immortalization of avian-derived cells.

Original languageEnglish
Pages (from-to)6709-6720
Number of pages12
JournalJournal of Cellular Physiology
Issue number5
Publication statusPublished - 2019 May
Externally publishedYes


  • avian
  • cell cycle
  • cellular senescence
  • endangered animal
  • immortalized cell

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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