Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

Kazuki Kato; Hiroshi Nishimasu; Emiko Mihara; Ryuichiro Ishitani; Junichi Takagi; Junken Aoki; Osamu Nureki

doi:10.1107/S1744309112019306

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

Kazuki Kato, Hiroshi Nishimasu, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Enpp1 is an extracellular membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing ATP to generate pyrophosphate. The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1^- cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X - ray diffraction data set was collected to 3.0 Å resolution. The crystal belonged to space group P31, with unit-cell parameters a = b = 105.3, c = 173.7 Å. A single-wavelength anomalous dispersion (SAD) data set was also collected to 2.7 Å resolution using a selenomethionine-labelled crystal. The experimental phases determined by the SAD method produced an interpretable electron-density map.

Original language	English
Pages (from-to)	778-782
Number of pages	5
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	68
Issue number	7
DOIs	https://doi.org/10.1107/S1744309112019306
Publication status	Published - 2012 Jul

Keywords

Enpp1

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1. / Kato, Kazuki; Nishimasu, Hiroshi; Mihara, Emiko; Ishitani, Ryuichiro; Takagi, Junichi; Aoki, Junken; Nureki, Osamu.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, No. 7, 07.2012, p. 778-782.

Research output: Contribution to journal › Article › peer-review

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