TY - JOUR
T1 - Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1
AU - Kato, Kazuki
AU - Nishimasu, Hiroshi
AU - Mihara, Emiko
AU - Ishitani, Ryuichiro
AU - Takagi, Junichi
AU - Aoki, Junken
AU - Nureki, Osamu
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - Enpp1 is an extracellular membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing ATP to generate pyrophosphate. The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1- cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X - ray diffraction data set was collected to 3.0 Å resolution. The crystal belonged to space group P31, with unit-cell parameters a = b = 105.3, c = 173.7 Å. A single-wavelength anomalous dispersion (SAD) data set was also collected to 2.7 Å resolution using a selenomethionine-labelled crystal. The experimental phases determined by the SAD method produced an interpretable electron-density map.
AB - Enpp1 is an extracellular membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing ATP to generate pyrophosphate. The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1- cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X - ray diffraction data set was collected to 3.0 Å resolution. The crystal belonged to space group P31, with unit-cell parameters a = b = 105.3, c = 173.7 Å. A single-wavelength anomalous dispersion (SAD) data set was also collected to 2.7 Å resolution using a selenomethionine-labelled crystal. The experimental phases determined by the SAD method produced an interpretable electron-density map.
KW - Enpp1
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U2 - 10.1107/S1744309112019306
DO - 10.1107/S1744309112019306
M3 - Article
C2 - 22750863
AN - SCOPUS:84863474643
VL - 68
SP - 778
EP - 782
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 7
ER -