Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones

Dengfeng Yang, Takahiro Mori, Takashi Matsui, Makoto Hashimoto, Hiroyuki Morita, Isao Fujii, Ikuro Abe

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6- yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P21, with unit-cell parameters a = 70.0, b = 104.8, c = 73.5 Å, β = 114.4°.

    Original languageEnglish
    Pages (from-to)730-733
    Number of pages4
    JournalActa Crystallographica Section F:Structural Biology Communications
    Volume70
    Issue number6
    DOIs
    Publication statusPublished - 2014 Jun

    Keywords

    • Aspergillus oryzae
    • CsyB
    • chalcone synthase
    • csypyrone B1
    • polyketide synthase

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Genetics
    • Condensed Matter Physics

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