Abstract
CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6- yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P21, with unit-cell parameters a = 70.0, b = 104.8, c = 73.5 Å, β = 114.4°.
Original language | English |
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Pages (from-to) | 730-733 |
Number of pages | 4 |
Journal | Acta Crystallographica Section F:Structural Biology Communications |
Volume | 70 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2014 Jun |
Externally published | Yes |
Keywords
- Aspergillus oryzae
- CsyB
- chalcone synthase
- csypyrone B1
- polyketide synthase
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics