Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones

Dengfeng Yang, Takahiro Mori, Takashi Matsui, Makoto Hashimoto, Hiroyuki Morita, Isao Fujii, Ikuro Abe

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6- yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P21, with unit-cell parameters a = 70.0, b = 104.8, c = 73.5 Å, β = 114.4°.

Original languageEnglish
Pages (from-to)730-733
Number of pages4
JournalActa Crystallographica Section F:Structural Biology Communications
Volume70
Issue number6
DOIs
Publication statusPublished - 2014 Jun
Externally publishedYes

Keywords

  • Aspergillus oryzae
  • CsyB
  • chalcone synthase
  • csypyrone B1
  • polyketide synthase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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