Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones

Dengfeng Yang; Takahiro Mori; Takashi Matsui; Makoto Hashimoto; Hiroyuki Morita; Isao Fujii; Ikuro Abe

doi:10.1107/S2053230X14008516

Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones

Dengfeng Yang, Takahiro Mori, Takashi Matsui, Makoto Hashimoto, Hiroyuki Morita, Isao Fujii, Ikuro Abe

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6- yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P21, with unit-cell parameters a = 70.0, b = 104.8, c = 73.5 Å, β = 114.4°.

Original language	English
Pages (from-to)	730-733
Number of pages	4
Journal	Acta Crystallographica Section F:Structural Biology Communications
Volume	70
Issue number	6
DOIs	https://doi.org/10.1107/S2053230X14008516
Publication status	Published - 2014 Jun

Keywords

Aspergillus oryzae
CsyB
chalcone synthase
csypyrone B1
polyketide synthase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones. / Yang, Dengfeng; Mori, Takahiro; Matsui, Takashi; Hashimoto, Makoto; Morita, Hiroyuki; Fujii, Isao; Abe, Ikuro.

In: Acta Crystallographica Section F:Structural Biology Communications, Vol. 70, No. 6, 06.2014, p. 730-733.

Research output: Contribution to journal › Article › peer-review

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abstract = "CsyB from Aspergillus oryzae is a novel type III polyketide synthase that catalyzes the formation of csypyrone B1 [4-(3-acetyl-4-hydroxy-2-oxo-2H-pyran-6- yl)butyric acid] from fatty acyl-CoA, malonyl-CoA and acetoacetyl-CoA. Recombinant CsyB expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space P21, with unit-cell parameters a = 70.0, b = 104.8, c = 73.5 {\AA}, β = 114.4°.",

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AU - Fujii, Isao

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