Expression profiling of novel bacteria-induced genes from the silkworm, Bombyx mori

Hiromitsu Tanaka, Noriko Suzuki, Yoshiro Nakajima, Masaaki Sato, Aki Sagisaka, Kosuke Fujita, Jun Ishibashi, Shigeo Imanishi, Kazuei Mita, Minoru Yamakawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, we have newly identified three bacteria-induced genes from the silkworm Bombyx mori by quantitative reverse transcriptasepolymerase chain reaction. One of these, eukaryotic initiation factor 4E-1 (eIF4E-1), is assumed to encode an eIF4E family, which plays a role in the initiation of translation as a mRNA cap-binding protein. The second gene is BmFOXG1, belonging to a family of forkhead transcription factors, FOXG1. The third gene is MBF2-related (MBF2-R) whose product has high homology to a co-activator protein MBF2 from B. mori. Although BmFOXG1 was up-regulated in the fat body in response to three kinds of bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, eIF4E-1 and MBF2-R were up-regulated by E. coli and B. subtilis, but not S. aureus, suggesting that bacteria possessing meso-diaminopimelic acid-containing peptidoglycan but not lysinecontaining peptidoglycan activate eIF4E-1 and MBF2-R, probably through a conserved immune deficiency pathway. We further profiled the expression of three genes in different tissues and a silkworm cell line, NIAS-Bm-aff3, in response to bacteria, and at different times after bacterial challenge in the fat body.

Original languageEnglish
Pages (from-to)148-162
Number of pages15
JournalArchives of Insect Biochemistry and Physiology
Volume73
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1
Externally publishedYes

Keywords

  • Bombyx mori
  • Forkhead transcription factor
  • Insect immunity
  • MBF2-related
  • eIF4E

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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  • Cite this

    Tanaka, H., Suzuki, N., Nakajima, Y., Sato, M., Sagisaka, A., Fujita, K., Ishibashi, J., Imanishi, S., Mita, K., & Yamakawa, M. (2010). Expression profiling of novel bacteria-induced genes from the silkworm, Bombyx mori. Archives of Insect Biochemistry and Physiology, 73(3), 148-162. https://doi.org/10.1002/arch.20347