Expression profile of plasmid DNA obtained using spermine derivatives of pullulan with different molecular weights

Jun Ichiro Jo, Tomonori Ikai, Arimichi Okazaki, Kentaro Nagane, Masaya Yamamoto, Yoshiaki Hirano, Yasuhiko Tabata

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The objective of this study was to prepare a novel gene carrier from pullulan, a polysaccharide with an inherent affinity for the liver, and evaluate the feasibility in gene transfection. Pullulan with different molecular weights was cationized by chemical introduction of spermine. The cationized pullulan derivative was complexed with a plasmid DNA and applied to HepG2 cells for in vitro gene transfection. The level of gene expression depended on the molecular weight of cationized pullulan derivatives and the highest level was observed for the cationized pullulan derivative with a molecular weight of 47.3 × 103. Pre-treatment of cells with asialofetuin decreased the level of gene expression by the complexes. These findings indicate that the cationized pullulan derivative is a promising non-viral carrier of plasmid DNA which is internalized in a receptor-mediated fashion.

Original languageEnglish
Pages (from-to)883-899
Number of pages17
JournalJournal of Biomaterials Science, Polymer Edition
Volume18
Issue number7
DOIs
Publication statusPublished - 2007 Jul
Externally publishedYes

Keywords

  • Asialoglycoprotein receptor
  • Gene expression
  • Molecular weight
  • Pullulan
  • Spermine

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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