Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells

Sabine Rütti, Caroline Arous, Alexandra C. Nica, Makoto Kanzaki, Philippe A. Halban, Karim Bouzakri

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The Rab-GTPase activating protein TBC1D1 is a paralog of AS160/TBC1D4. AS160/TBC1D4, a downstream effector of Akt, has been shown to play a central role in beta-cell function and survival. The two proteins have overlapping function in insulin signalling in muscle cells. However, the expression and the potential role of TBC1D1 in beta-cells remain unknown. Therefore, the aim of this study is to investigate whether TBC1D1 is expressed in beta-cells and whether it plays, as AS160/TBC1D4, a role in beta-cell function and survival. Using human and rat beta-cells, this study shows for the first time that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. Knockdown of TBC1D1 in beta-cells resulted in increased basal and glucose-stimulated insulin release, decreased proliferation but no change in apoptosis.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalFEBS Letters
Volume588
Issue number1
DOIs
Publication statusPublished - 2014 Jan 3

Keywords

  • Apoptosis
  • Beta-cell
  • Glucose response
  • Proliferation
  • TBC1D1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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