Expression of voltage-dependent chloride channels in the rat cochlea

Takeshi Oshima, Katsuhisa Ikeda, Masayuki Furukawa, Tomonori Takasaka

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Voltage-dependent chloride (ClC) channels have not yet been identified in the cochlea. In this study, an approach utilizing the reverse transcription-polymerase chain reaction (RT-RCR) was devised to clone the cDNA of ClC channels. PCR was performed using degenerate primers corresponding to two highly conserved regions of the ClC channels. By Southern hybridization and sequencing studies, the sequences corresponding to ClC-2 and ClC-3 were found in the cochlear lateral wall, while ClC-1 was not detected. These results suggest that ClC-2 and ClC-3 might be involved in Cl- transport in the cochlear lateral wall.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalHearing Research
Volume103
Issue number1-2
DOIs
Publication statusPublished - 1997 Jan

Keywords

  • RT-PCR
  • chloride channel
  • cochlea
  • rat
  • voltage-dependent chloride channel

ASJC Scopus subject areas

  • Sensory Systems

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