Expression of V-1, a novel catecholamine biosynthesis regulatory protein, is enhanced by hypertension in atrial myocytes of Dahl salt-sensitive rats

Tohru Yamakuni, Michio Hashimoto, Hiroyuki Sakagami, Toshifumi Yamamoto, Masumi Kobayashi, Yoshimi Fujii, Hideko Yamamoto, Dileep Kumar Rohra, Yusuke Hiwatashi, Takao Honma, Hisatake Kondo, Osamu Shido, Yasushi Ohizumi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

V-1 positively controls catecholamine synthetic gene transcription to promote catecholamine production in PC12D cells. In this study, immunohistochemical analysis revealed that in Wistar rats, V-1 immunoreactivity was localized not only in sympathetic axons but also in the cytoplasm of cardiomyocytes, and that the immunoreactivity in atrial myocytes was more intense than that in ventricular myocytes. Western blot analysis also showed that V-1 expression level in the atrium was higher than that in the ventricle of Wistar rat hearts. When Dahl salt-sensitive (DS) rats were fed an 8% NaCl diet after the age of 6 weeks, blood pressure was raised 230 mm Hg at 18 weeks. V-1 expression was shown to be increased in the atrial myocytes of these DS rats, but not in the sympathetic axons, when assayed by immunohistochemistry. These results suggest that in normotensive rats, V-1 is preferentially expressed in the cytoplasm of cardiomyocytes in the atrium rather than in the ventricle. It is also suggested that V-1 expression is increased by hypertension in DS rat atrium.

Original languageEnglish
Pages (from-to)793-797
Number of pages5
JournalBiochemical and biophysical research communications
Volume298
Issue number5
DOIs
Publication statusPublished - 2002

Keywords

  • Cardiomyocyte
  • Catecholamine biosynthesis
  • Dahl salt-sensitive rat
  • Hypertension
  • V-1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Yamakuni, T., Hashimoto, M., Sakagami, H., Yamamoto, T., Kobayashi, M., Fujii, Y., Yamamoto, H., Rohra, D. K., Hiwatashi, Y., Honma, T., Kondo, H., Shido, O., & Ohizumi, Y. (2002). Expression of V-1, a novel catecholamine biosynthesis regulatory protein, is enhanced by hypertension in atrial myocytes of Dahl salt-sensitive rats. Biochemical and biophysical research communications, 298(5), 793-797. https://doi.org/10.1016/S0006-291X(02)02529-9