TY - JOUR
T1 - Expression of V-1, a novel catecholamine biosynthesis regulatory protein, is enhanced by hypertension in atrial myocytes of Dahl salt-sensitive rats
AU - Yamakuni, Tohru
AU - Hashimoto, Michio
AU - Sakagami, Hiroyuki
AU - Yamamoto, Toshifumi
AU - Kobayashi, Masumi
AU - Fujii, Yoshimi
AU - Yamamoto, Hideko
AU - Rohra, Dileep Kumar
AU - Hiwatashi, Yusuke
AU - Honma, Takao
AU - Kondo, Hisatake
AU - Shido, Osamu
AU - Ohizumi, Yasushi
N1 - Funding Information:
We thank Dr. Yasuo Takahashi for a critical reading of the manuscript and discussion. This work was supported in part by Grants-in-Aid for Scientific Research from The Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2002
Y1 - 2002
N2 - V-1 positively controls catecholamine synthetic gene transcription to promote catecholamine production in PC12D cells. In this study, immunohistochemical analysis revealed that in Wistar rats, V-1 immunoreactivity was localized not only in sympathetic axons but also in the cytoplasm of cardiomyocytes, and that the immunoreactivity in atrial myocytes was more intense than that in ventricular myocytes. Western blot analysis also showed that V-1 expression level in the atrium was higher than that in the ventricle of Wistar rat hearts. When Dahl salt-sensitive (DS) rats were fed an 8% NaCl diet after the age of 6 weeks, blood pressure was raised 230 mm Hg at 18 weeks. V-1 expression was shown to be increased in the atrial myocytes of these DS rats, but not in the sympathetic axons, when assayed by immunohistochemistry. These results suggest that in normotensive rats, V-1 is preferentially expressed in the cytoplasm of cardiomyocytes in the atrium rather than in the ventricle. It is also suggested that V-1 expression is increased by hypertension in DS rat atrium.
AB - V-1 positively controls catecholamine synthetic gene transcription to promote catecholamine production in PC12D cells. In this study, immunohistochemical analysis revealed that in Wistar rats, V-1 immunoreactivity was localized not only in sympathetic axons but also in the cytoplasm of cardiomyocytes, and that the immunoreactivity in atrial myocytes was more intense than that in ventricular myocytes. Western blot analysis also showed that V-1 expression level in the atrium was higher than that in the ventricle of Wistar rat hearts. When Dahl salt-sensitive (DS) rats were fed an 8% NaCl diet after the age of 6 weeks, blood pressure was raised 230 mm Hg at 18 weeks. V-1 expression was shown to be increased in the atrial myocytes of these DS rats, but not in the sympathetic axons, when assayed by immunohistochemistry. These results suggest that in normotensive rats, V-1 is preferentially expressed in the cytoplasm of cardiomyocytes in the atrium rather than in the ventricle. It is also suggested that V-1 expression is increased by hypertension in DS rat atrium.
KW - Cardiomyocyte
KW - Catecholamine biosynthesis
KW - Dahl salt-sensitive rat
KW - Hypertension
KW - V-1
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U2 - 10.1016/S0006-291X(02)02529-9
DO - 10.1016/S0006-291X(02)02529-9
M3 - Article
C2 - 12419325
AN - SCOPUS:0036432932
VL - 298
SP - 793
EP - 797
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 5
ER -