TY - JOUR
T1 - Expression of tumor necrosis factor-α in muscles of polymyositis
AU - Tateyama, M.
AU - Nagano, I.
AU - Yoshioka, M.
AU - Chida, K.
AU - Nakamura, S.
AU - Itoyama, Y.
N1 - Funding Information:
We wish to thank staff of the Department of Neurology at Tohoku university for providing muscle samples. We wish to thank Dr. Kazuo Kobayashi for histological evaluation and Ms. Rumiko Tanaka for technical assistance. This work was supported by a grant from the Immunoneurological Diseases Research Committee, the Ministry of Health and Welfare of Japan and a grant-in-aid for scientific research (C-05670544) from the Ministry of Education, Science and Culture of Japan.
PY - 1997/2/27
Y1 - 1997/2/27
N2 - We immunohistochemically examined biopsied muscles from nine untreated patients with polymyositis (PM) and five patients with other neuromuscular diseases (ONMD), using monoclonal antibodies to tumor necrosis factor-α (TNF-α) and lymphoid surface markers. In muscles of three patients with PM, we observed many TNF-α positive macrophages and lymphocytes in endomysium and around vessels in the muscles. By contrast, there were few, weakly TNF-α stained cells in muscles of three patients with ONMD. The ratio of TNF-α-positive cells to the muscle fibers and the ratio of TNF-α-positive cells to the mononuclear cells were significantly higher in PM compared with ONMD. In addition, we obsered atrophic muscle fibers more frequently in TNF-α-positive muscles than TNF-α-negative ones. We conclude that, at least, in a part of PM patients, TNF-α produced locally may contribute to the pathogenesis of PM.
AB - We immunohistochemically examined biopsied muscles from nine untreated patients with polymyositis (PM) and five patients with other neuromuscular diseases (ONMD), using monoclonal antibodies to tumor necrosis factor-α (TNF-α) and lymphoid surface markers. In muscles of three patients with PM, we observed many TNF-α positive macrophages and lymphocytes in endomysium and around vessels in the muscles. By contrast, there were few, weakly TNF-α stained cells in muscles of three patients with ONMD. The ratio of TNF-α-positive cells to the muscle fibers and the ratio of TNF-α-positive cells to the mononuclear cells were significantly higher in PM compared with ONMD. In addition, we obsered atrophic muscle fibers more frequently in TNF-α-positive muscles than TNF-α-negative ones. We conclude that, at least, in a part of PM patients, TNF-α produced locally may contribute to the pathogenesis of PM.
KW - immunohistochemistry
KW - lymphocyte subsets
KW - polymyositis
KW - tumor necrosis factor-α
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U2 - 10.1016/S0022-510X(96)00280-8
DO - 10.1016/S0022-510X(96)00280-8
M3 - Article
C2 - 9077495
AN - SCOPUS:0031049412
VL - 146
SP - 45
EP - 51
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 1
ER -