TY - JOUR
T1 - Expression of thyroid hormone receptor βA gene assayed by transgenic Xenopus laevis carrying its promoter sequences
AU - Oofusa, Ken
AU - Tooi, Osamu
AU - Kashiwagi, Akihiko
AU - Kashiwagi, Keiko
AU - Kondo, Yasuyuki
AU - Watanabe, Yusuke
AU - Sawada, Takuya
AU - Fujikawa, Kazuyuki
AU - Yoshizato, Katsutoshi
N1 - Funding Information:
The authors wish to thank Dr Y.-B. Shi for his kind gift of pCAT-WT plasmid. A part of this study was supported by a Grant-in-Aid for Scientific Research (A), #07404057, from the Ministry of Education, Science, Sports, and Culture of Japan. We also thank Shiono Hiratsu and Akiko Nakahata for their excellent technical assistance.
PY - 2001/7/5
Y1 - 2001/7/5
N2 - The responsiveness of thyroid hormone responsive element (TRE)-containing promoter sequence to thyroid hormone (TH) was studied utilizing Xenopus laevis carrying a transgene containing 5′-upstream region of TRβA1 gene and green fluorescent protein (EGFP) gene. EGFP-expression was seen first in neurulae, which continued to stage 45, then became weak, and again started to increase at the prometamorphic stage, culminating at the metamorphic climax stage. Immunohistochemistry identified eyes, viscera, and muscles as the EGFP-expressing larval tissues. The treatment of premetamorphic tadpoles with TH induced the precocious EGFP-expression. We also showed that the transgenic Xenopus adults were responsive to exogenous TH, a high responsiveness being seen in brain, small intestine, kidney, and bone. TRβ-expression in the embryo, larva, and adult was verified by Western blotting. Thus, TH not only regulates the metamorphosis, but also might play some biological role(s) in embryos and adults.
AB - The responsiveness of thyroid hormone responsive element (TRE)-containing promoter sequence to thyroid hormone (TH) was studied utilizing Xenopus laevis carrying a transgene containing 5′-upstream region of TRβA1 gene and green fluorescent protein (EGFP) gene. EGFP-expression was seen first in neurulae, which continued to stage 45, then became weak, and again started to increase at the prometamorphic stage, culminating at the metamorphic climax stage. Immunohistochemistry identified eyes, viscera, and muscles as the EGFP-expressing larval tissues. The treatment of premetamorphic tadpoles with TH induced the precocious EGFP-expression. We also showed that the transgenic Xenopus adults were responsive to exogenous TH, a high responsiveness being seen in brain, small intestine, kidney, and bone. TRβ-expression in the embryo, larva, and adult was verified by Western blotting. Thus, TH not only regulates the metamorphosis, but also might play some biological role(s) in embryos and adults.
KW - Anuran metamorphosis
KW - Promoter assay
KW - Thyroid hormone responsive element
KW - Transgenic Xenopus
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U2 - 10.1016/S0303-7207(01)00529-9
DO - 10.1016/S0303-7207(01)00529-9
M3 - Article
C2 - 11476944
AN - SCOPUS:0035811517
VL - 181
SP - 97
EP - 110
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -