Expression of thyroid hormone receptor βA gene assayed by transgenic Xenopus laevis carrying its promoter sequences

Ken Oofusa, Osamu Tooi, Akihiko Kashiwagi, Keiko Kashiwagi, Yasuyuki Kondo, Yusuke Watanabe, Takuya Sawada, Kazuyuki Fujikawa, Katsutoshi Yoshizato

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

The responsiveness of thyroid hormone responsive element (TRE)-containing promoter sequence to thyroid hormone (TH) was studied utilizing Xenopus laevis carrying a transgene containing 5′-upstream region of TRβA1 gene and green fluorescent protein (EGFP) gene. EGFP-expression was seen first in neurulae, which continued to stage 45, then became weak, and again started to increase at the prometamorphic stage, culminating at the metamorphic climax stage. Immunohistochemistry identified eyes, viscera, and muscles as the EGFP-expressing larval tissues. The treatment of premetamorphic tadpoles with TH induced the precocious EGFP-expression. We also showed that the transgenic Xenopus adults were responsive to exogenous TH, a high responsiveness being seen in brain, small intestine, kidney, and bone. TRβ-expression in the embryo, larva, and adult was verified by Western blotting. Thus, TH not only regulates the metamorphosis, but also might play some biological role(s) in embryos and adults.

Original languageEnglish
Pages (from-to)97-110
Number of pages14
JournalMolecular and Cellular Endocrinology
Volume181
Issue number1-2
DOIs
Publication statusPublished - 2001 Jul 5

Keywords

  • Anuran metamorphosis
  • Promoter assay
  • Thyroid hormone responsive element
  • Transgenic Xenopus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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