Expression of the pre-S(2) gene of hepatitis B virus in Escherichia coli

K. Tachibana; M. Imai; H. Okamoto; K. Yoda; H. Kadokura; Y. Katayama; M. Yamasaki

doi:10.1007/BF01032560

Expression of the pre-S(2) gene of hepatitis B virus in Escherichia coli

K. Tachibana, M. Imai, H. Okamoto, K. Yoda, H. Kadokura, Y. Katayama, M. Yamasaki

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The pre-S(2) antigen of hepatitis B virus was produced as a fused protein with the E.coli alkaline phosphatase. The product remained in precipitable materials but could be solubilized by 8M urea treatment. The amount of the bacterial antigen was equivalent to 160 μg of serum HBsAg per ml of culture.

Original language	English
Pages (from-to)	677-682
Number of pages	6
Journal	Biotechnology Letters
Volume	8
Issue number	10
DOIs	https://doi.org/10.1007/BF01032560
Publication status	Published - 1986 Oct 1
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/BF01032560

Cite this

@article{281456cff6d34e0097ef8692b6b2dcd9,

title = "Expression of the pre-S(2) gene of hepatitis B virus in Escherichia coli",

abstract = "The pre-S(2) antigen of hepatitis B virus was produced as a fused protein with the E.coli alkaline phosphatase. The product remained in precipitable materials but could be solubilized by 8M urea treatment. The amount of the bacterial antigen was equivalent to 160 μg of serum HBsAg per ml of culture.",

author = "K. Tachibana and M. Imai and H. Okamoto and K. Yoda and H. Kadokura and Y. Katayama and M. Yamasaki",

year = "1986",

month = oct,

day = "1",

doi = "10.1007/BF01032560",

language = "English",

volume = "8",

pages = "677--682",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "10",

}

TY - JOUR

T1 - Expression of the pre-S(2) gene of hepatitis B virus in Escherichia coli

AU - Tachibana, K.

AU - Imai, M.

AU - Okamoto, H.

AU - Yoda, K.

AU - Kadokura, H.

AU - Katayama, Y.

AU - Yamasaki, M.

PY - 1986/10/1

Y1 - 1986/10/1

N2 - The pre-S(2) antigen of hepatitis B virus was produced as a fused protein with the E.coli alkaline phosphatase. The product remained in precipitable materials but could be solubilized by 8M urea treatment. The amount of the bacterial antigen was equivalent to 160 μg of serum HBsAg per ml of culture.

AB - The pre-S(2) antigen of hepatitis B virus was produced as a fused protein with the E.coli alkaline phosphatase. The product remained in precipitable materials but could be solubilized by 8M urea treatment. The amount of the bacterial antigen was equivalent to 160 μg of serum HBsAg per ml of culture.

UR - http://www.scopus.com/inward/record.url?scp=34250131307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250131307&partnerID=8YFLogxK

U2 - 10.1007/BF01032560

DO - 10.1007/BF01032560

M3 - Article

AN - SCOPUS:34250131307

SN - 0141-5492

VL - 8

SP - 677

EP - 682

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 10

ER -

Expression of the pre-S(2) gene of hepatitis B virus in Escherichia coli

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this