Expression of the IL-2 receptor γ chain on various populations in human peripheral blood

Naoto Ishil, Toshikazu Takeshita, Yutaka Kimura, Kohtaro Tada, Motonari Kondo, Masataka Nakamura, Kazuo Sugamura

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)

Abstract

We have established two rat mAbs, TUGH4 and TUGh5, specific for the human γ chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human γ chain gene but not to their parental cell lines, and precipitated 65-70 and 80-90 kDa cell surface molecules from lysates of human T cells surface-labeled with Na125I and chemically cross-linked with [125]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the γ chain in a wide variety of peripheral blood cell populations including CD4+ T cells, CD20+ T cells, CD20+ B cells, CD56+ natural killer cells, CD4+ monocytes and granulocytes, contrasting with expression of the α and β chains of IL-2R.

Original languageEnglish
Pages (from-to)1273-1277
Number of pages5
JournalInternational immunology
Volume6
Issue number8
DOIs
Publication statusPublished - 1994 Aug

Keywords

  • IL-2
  • IL-2 receptor γ chain
  • Monoclonal antibody
  • Peripheral blood

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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