TY - JOUR
T1 - Expression of the IL-2 receptor γ chain on various populations in human peripheral blood
AU - Ishil, Naoto
AU - Takeshita, Toshikazu
AU - Kimura, Yutaka
AU - Tada, Kohtaro
AU - Kondo, Motonari
AU - Nakamura, Masataka
AU - Sugamura, Kazuo
N1 - Funding Information:
We thank Japanese Cancer Research Resources Bank for a cell line BW5147 and J. Hamuro for recombinant IL-2. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture, by the Special Coordination Funds of the Science and Technology Agency, by a Grant-in-Aid from the Ministry of Health and Welfare of the Japanese Government from the Comprehensive Ten-Year Strategy for Cancer Control, by a Grant from the Mochida Memorial Foundation for Medical and Pharmaceutical Research, and by the Nalto Foundation.
PY - 1994/8
Y1 - 1994/8
N2 - We have established two rat mAbs, TUGH4 and TUGh5, specific for the human γ chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human γ chain gene but not to their parental cell lines, and precipitated 65-70 and 80-90 kDa cell surface molecules from lysates of human T cells surface-labeled with Na125I and chemically cross-linked with [125]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the γ chain in a wide variety of peripheral blood cell populations including CD4+ T cells, CD20+ T cells, CD20+ B cells, CD56+ natural killer cells, CD4+ monocytes and granulocytes, contrasting with expression of the α and β chains of IL-2R.
AB - We have established two rat mAbs, TUGH4 and TUGh5, specific for the human γ chain of the IL-2 receptor (IL-2R), which is known to be shared among receptors for IL-2, IL-4 and IL-7. The antibodies bound to cell lines transfected with the human γ chain gene but not to their parental cell lines, and precipitated 65-70 and 80-90 kDa cell surface molecules from lysates of human T cells surface-labeled with Na125I and chemically cross-linked with [125]IL-2 respectively. Flow cytometry with TUGh4 and TUGh5 detected the γ chain in a wide variety of peripheral blood cell populations including CD4+ T cells, CD20+ T cells, CD20+ B cells, CD56+ natural killer cells, CD4+ monocytes and granulocytes, contrasting with expression of the α and β chains of IL-2R.
KW - IL-2
KW - IL-2 receptor γ chain
KW - Monoclonal antibody
KW - Peripheral blood
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U2 - 10.1093/intimm/6.8.1273
DO - 10.1093/intimm/6.8.1273
M3 - Article
C2 - 7981154
AN - SCOPUS:0028023210
VL - 6
SP - 1273
EP - 1277
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 8
ER -