TY - JOUR
T1 - Expression of the HT462 antigen on fresh leukemic T cells and on cells of HTLV-I infected lines
AU - Hattori, Toshio
AU - Matsuoka, Masao
AU - Chosa, Toru
AU - Yoshiki, Takashi
AU - Robert-Guroff, Marjorie
AU - Takatsuki, Kiyoshi
N1 - Funding Information:
Acknowledgements--This work was partly supported by Grant-in Aid (No. 61570585) from the Ministry of Education, Science and Culture of Japan. We are grateful to Drs I. Miyoshi and M. Yoshida for generous supply of MT-2 cells and demonstration of proviral DNA of HTLV-I in ATL cells, respectively. The authors are indebted for Dr F. Wong-Staal for providing us her unpublished data. We are also grateful to Dr R. C. Gallo for continued encouragement.
PY - 1987
Y1 - 1987
N2 - We have examined expression of antigens defined by HT462 monoclonal antibody (mAb), together with other HTLV-I related antigens using phorbol 12-myristate 13-acetate treated leukemic mature T cells. Thirteen patients with adult T-cell leukemia (ATL), 3 patients in remission states of ATL and 5 patients with non-ATL were examined. All ATL cells expressed the HT462 antigen, however cells from patients in remission did not express the HT462 antigen. A low percentage of cells from 2 out of 5 patients with non-ATL mature leukemic T cells expressed the HT462 antigen, although these cells did not express other HTLV-I related antigens. Cells of HTLV-I infected human cell lines expressed the HT462 antigen. Three HTLV-I infected rat cell lines (TARS-1, TART-1, TARL-2) did not express the HT462 antigen, although cells of these lines expressed other HTLV-I related antigens. Characterization of the HT462 antigen by strip radioimmunoassay based on western blotting technique using cell lysates of HUT102 cells revealed two additional bands (p68, p35) together with previously reported proteins (gp52, p42). Only p68 was seen in western blots using cell lysates of the rat cell lines. These findings further suggest that the HT462 antigen is a cellular component induced in virus transformed human cells and not a virus encoded protein.
AB - We have examined expression of antigens defined by HT462 monoclonal antibody (mAb), together with other HTLV-I related antigens using phorbol 12-myristate 13-acetate treated leukemic mature T cells. Thirteen patients with adult T-cell leukemia (ATL), 3 patients in remission states of ATL and 5 patients with non-ATL were examined. All ATL cells expressed the HT462 antigen, however cells from patients in remission did not express the HT462 antigen. A low percentage of cells from 2 out of 5 patients with non-ATL mature leukemic T cells expressed the HT462 antigen, although these cells did not express other HTLV-I related antigens. Cells of HTLV-I infected human cell lines expressed the HT462 antigen. Three HTLV-I infected rat cell lines (TARS-1, TART-1, TARL-2) did not express the HT462 antigen, although cells of these lines expressed other HTLV-I related antigens. Characterization of the HT462 antigen by strip radioimmunoassay based on western blotting technique using cell lysates of HUT102 cells revealed two additional bands (p68, p35) together with previously reported proteins (gp52, p42). Only p68 was seen in western blots using cell lysates of the rat cell lines. These findings further suggest that the HT462 antigen is a cellular component induced in virus transformed human cells and not a virus encoded protein.
KW - Adult T-cell leukemia
KW - HT462 antigen
KW - human T-cell lymphotropic virus
KW - monoclonal antibody
UR - http://www.scopus.com/inward/record.url?scp=0023098436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023098436&partnerID=8YFLogxK
U2 - 10.1016/0145-2126(87)90048-8
DO - 10.1016/0145-2126(87)90048-8
M3 - Article
C2 - 2882067
AN - SCOPUS:0023098436
SN - 0145-2126
VL - 11
SP - 251
EP - 257
JO - Leukemia Research
JF - Leukemia Research
IS - 3
ER -
