Expression of taste reception response of fleshfly in Xenopus oocytes

Masayuki Koganezawa, Ryuzo Shingai, Kunio Isono, Ichiro Shimada

Research output: Contribution to journalArticlepeer-review

Abstract

Functional expression of the gustatory sensitivity to amino acids and sugars was investigated in Xenopus oocytes injected with poly(A+) RNA extracted from fleshfly labellar taste organs. The current induced by the application of amino acids and sugars that stimulate fleshfly sugar receptor cells was recorded under voltage clamp conditions. L-Phenylalanine and L-valine induced a large transient inward current in poly(A+) RNA-injected oocytes but only small responses in uninjected control oocytes. Glucose and fructose also produced inward currents in the RNA-injected oocytes. N-methylated compounds or D-isomers of these amino acids induced either no response or a much smaller response than L-amino acids as they did in the fleshfly sugar receptor cells. The oocyte expression system is a useful tool for characterizing the taste transduction mechanism.

Original languageEnglish
Pages (from-to)2063-2067
Number of pages5
JournalNeuroReport
Volume7
Issue number13
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • Amino acids and sugars
  • Fleshfly
  • Ionic currents
  • Poly(A+) RNA
  • Taste receptor
  • Xenopus oocyte

ASJC Scopus subject areas

  • Neuroscience(all)

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