Expression of tac antigen on human immature B-cell lineage leukemic cells

Masao Matsuoka, Toshio Hattori, Fumio Kawano, Toshinori Ishii, Takashi Uchiyama, Kiyoshi Takatsuki

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12 Citations (Scopus)

Abstract

Expression of interleukin 2 (IL-2) receptor (Tac antigen) on leukemic cells from 4 patients with acute lymphocytic leukemia (ALL) and 3 patients with blastic crisis of chronic myelogenous leukemia (CML-BC) was examined. Cells from 6 out of 7 patients did not carry immunoglobulins on their surfaces, but reacted with monoclonal antibodies (mAb) detecting B-cell related antigens such as B1, OKB2 and Leu12. Cells from two cases expressed Tac antigen immediately after cell separation. Phorbol 12-myristate 13-acetate (PMA) could induce Tac antigen in all cases. SDS-PAGE analysis of immunoprecipitates by anti-Tac mAb demonstrated that the molecular weight of Tac antigen on most of the leukemic cells was similar to that of Tac antigen present on Con A-stimulated normal lymphocytes. These leukemic cells poorly responded to exogenous recombinant IL-2. These findings suggest that IL-2 may interact with the immature and mature B cells, and play an important role in the differentiation of B lymphocytes.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalLeukemia Research
Volume10
Issue number6
DOIs
Publication statusPublished - 1986

Keywords

  • IL-2 receptor
  • Tac antigen
  • acute lymphocytic leukemia
  • interleukin-2
  • phorbol ester

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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  • Cite this

    Matsuoka, M., Hattori, T., Kawano, F., Ishii, T., Uchiyama, T., & Takatsuki, K. (1986). Expression of tac antigen on human immature B-cell lineage leukemic cells. Leukemia Research, 10(6), 597-603. https://doi.org/10.1016/0145-2126(86)90261-4