Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes

Tomokazu Fukuda, Tetsuya Tani, Seiki Haraguchi, Kenichiro Donai, Nobuyoshi Nakajima, Hirohide Uenishi, Takahiro Eitsuka, Makoto Miyagawa, Sanghoun Song, Manabu Onuma, Yumi Hoshino, Eimei Sato, Arata Honda

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    In this study, we created porcine-induced pluripotent stem (iPS) cells with the expression of six reprogramming factors (Oct3/4, Klf4, Sox2, c-Myc, Lin28, and Nanog). The resulting cells showed growth dependent on LIF (leukemia inhibitory factor) and expression of multiple stem cell markers. Furthermore, the iPS cells caused teratoma formation with three layers of differentiation and had both active X chromosomes (XaXa). Our iPS cells satisfied the both of important characteristics of stem cells: teratoma formation and activation of both X chromosomes. Injection of these iPS cells into morula stage embryos showed that these cells participate in the early stage of porcine embryogenesis. Furthermore, the RNA-Seq analysis detected that expression levels of endogenous pluripotent related genes, NANOG, SOX2, ZFP42, OCT3/4, ESRRB, and ERAS were much higher in iPS with six factors than that with four reprogramming factors. We can conclude that the expression of six reprogramming factors enables the creation of porcine iPS cells, which is partially close to naive iPS state. J. Cell. Biochem. 118: 537–553, 2017.

    Original languageEnglish
    Pages (from-to)537-553
    Number of pages17
    JournalJournal of Cellular Biochemistry
    Volume118
    Issue number3
    DOIs
    Publication statusPublished - 2017 Mar 1

    Keywords

    • EPIGENETIC STATUS
    • PLURIPOTENT STEM CELL
    • PORCINE
    • TRANSCRIPTIONAL FACTORS
    • X CHROMOSOME

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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