Expression of rat protein phosphatase 2C (IA) in Escherichia coli

Shinri tamura, Akira Yasui, Shigeru Tsuiki

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    A cDNA containing the entire coding sequence of rat type 2C (IA) protein phosphatase was expressed in Escherichia coli. An extract of bacterial cells harboring the recombinant plasmid contained a major (Mr = 41,000 - 4,3,000) and a minor (Mr = 30,000) protein band; both of these reacted with an anti-type 2C protein phosphatase serum. The size of the major protein band agrees well with that of the 2C phosphatase conceptualized from the cognate cDNA. A Mg2+-dependent protein phosphatase activity was detected in extracts containing the recombinant protein, but not in host cell extracts. Based on these results, it is concluded that the isolated cDNA clone encodes a functional type 2C protein phosphatase.

    Original languageEnglish
    Pages (from-to)131-136
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume163
    Issue number1
    DOIs
    Publication statusPublished - 1989 Aug 30

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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