TY - JOUR
T1 - Expression of rat protein phosphatase 2C (IA) in Escherichia coli
AU - tamura, Shinri
AU - Yasui, Akira
AU - Tsuiki, Shigeru
PY - 1989/8/30
Y1 - 1989/8/30
N2 - A cDNA containing the entire coding sequence of rat type 2C (IA) protein phosphatase was expressed in Escherichia coli. An extract of bacterial cells harboring the recombinant plasmid contained a major (Mr = 41,000 - 4,3,000) and a minor (Mr = 30,000) protein band; both of these reacted with an anti-type 2C protein phosphatase serum. The size of the major protein band agrees well with that of the 2C phosphatase conceptualized from the cognate cDNA. A Mg2+-dependent protein phosphatase activity was detected in extracts containing the recombinant protein, but not in host cell extracts. Based on these results, it is concluded that the isolated cDNA clone encodes a functional type 2C protein phosphatase.
AB - A cDNA containing the entire coding sequence of rat type 2C (IA) protein phosphatase was expressed in Escherichia coli. An extract of bacterial cells harboring the recombinant plasmid contained a major (Mr = 41,000 - 4,3,000) and a minor (Mr = 30,000) protein band; both of these reacted with an anti-type 2C protein phosphatase serum. The size of the major protein band agrees well with that of the 2C phosphatase conceptualized from the cognate cDNA. A Mg2+-dependent protein phosphatase activity was detected in extracts containing the recombinant protein, but not in host cell extracts. Based on these results, it is concluded that the isolated cDNA clone encodes a functional type 2C protein phosphatase.
UR - http://www.scopus.com/inward/record.url?scp=0024435007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024435007&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)92109-8
DO - 10.1016/0006-291X(89)92109-8
M3 - Article
C2 - 2549985
AN - SCOPUS:0024435007
VL - 163
SP - 131
EP - 136
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -