Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion

Chika Saegusa, Eiko Kanno, Shigeyoshi Itohara, Mitsunori Fukuda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Slp1 is a putative Rab27 effector protein and implicated in intracellular membrane transport; however, the precise tissue distribution and function of Slp1 protein remain largely unknown. In this study we investigated the tissue distribution of Slp1 in mice and found that Slp1 is abundantly expressed in the pancreas, especially in the apical region of pancreatic acinar cells. Slp1 interacted with Rab27B in vivo and both proteins were co-localized on zymogen granules. Morphological analysis of fasted Slp1 knockout mice showed an increased number of zymogen granules in the pancreatic acinar cells, indicating that Slp1 is part of the machinery of amylase secretion by the exocrine pancreas.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume475
Issue number1
DOIs
Publication statusPublished - 2008 Jul 1

Keywords

  • Knockout mice
  • Pancreatic acini
  • Rab27 effector
  • Slp1
  • Zymogen granule

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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