Expression of (pro)renin receptor in human erythroid cell lines and its increased protein accumulation by interferon-γ

Kiriko Kaneko, Hiroshi Nishiyama, Koji Ohba, Akiko Shibasaki, Takuo Hirose, Kazuhito Totsune, Kazumichi Furuyama, Kazuhiro Takahashi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The renin-angiotensin system is known to enhance erythropoiesis. (Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, has recently been identified. However, expression of (P)RR in erythroid cells has not been studied. The aim of the present study is to clarify expression of (P)RR in erythroid cells, and the effects of erythropoietin, angiotensin II, transforming growth factor-β1 (TGF-β1), interferon-γ (IFN-γ) and interleukin-1β (IL-1β) on its expression. Western blot analysis showed that (P)RR protein was expressed in human cultured erythroid cell lines, YN-1 and YN-1-0-A (a clonal variant cell line of YN-1). Erythropoietin (1 IU/ml) increased (P)RR mRNA expression levels in YN-1-0-A cells (1.7-fold increase compared with control), but angiotensin II did not. Treatment of YN-1-0-A cells with IFN-γ (10 ng/ml) for 48 h increased the expression levels of (P)RR protein significantly (1.4-fold increase compared with control), whereas it had no significant effects on expression levels of (P)RR mRNA. Treatment of YN-1-0-A cells with TGF-β1 or IL-1β for 24 or 48 h had no significant effects on expression levels of (P)RR. The present study has shown for the first time expression of (P)RR in erythroid cells, raising the possibility that (P)RR may have a role in erythropoiesis and the pathophysiology of certain types of anemia.

Original languageEnglish
Pages (from-to)285-289
Number of pages5
JournalPeptides
Volume37
Issue number2
DOIs
Publication statusPublished - 2012 Oct

Keywords

  • (Pro)renin receptor
  • Angiotensin
  • Cytokine
  • Erythroid
  • Erythropoiesis
  • Erythropoietin
  • Interferon
  • Prorenin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Expression of (pro)renin receptor in human erythroid cell lines and its increased protein accumulation by interferon-γ'. Together they form a unique fingerprint.

Cite this