Expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase in human bladder cancer

Tsutomu Tanaka, Tatsuhiro Yoshiki, Yoichi Arai, Kayoko Higuchi, Susumu Kageyama, Yasutaka Ogawa, Takahiro Isono, Yusaku Okada

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We investigated the expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) in primary bladder cancer, its association with clinicopathologic findings, and their prognostic value. mRNA was extracted from 20 bladder cancer specimens and 6 normal bladder mucosal tissues. Relative amounts of PD-ECGF/TP mRNA were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and compared with the level of glyceraldehyde-3-phosphate dehydrogenase mRNA (used as an internal standard). PD-ECGF/TP expression was examined by immunohistochemistry in 85 patients who underwent cystectomy for bladder cancer. Serum PD-ECGF/TP levels were measured in 23 patients using a sandwich-type enzyme-linked immunosorbent assay. By RT-PCR analysis, expression of PD-ECGF/TP was found to be 7-fold higher in invasive tumors than in superficial tumors (P < 0.01) and 9-fold higher than in normal bladder (P < 0.01). Out of 85 transitional cell carcinoma tissue samples, 69 (81%) were evaluated as PD-ECGF/TP-positive by immunohistochemical staining. PD-ECGF/TP expression correlated significantly with tumor grade (P = 0.001), depth of invasion (P = 0.012), and lymphatic invasion (P = 0.01). No correlation was found between expression of PD-ECGF/TP and the number of tumors, tumor configuration, lymph node involvement, venous invasion, c-erbB-2 expression, or overall survival. We could not detect a significant serum level of PD-ECGF/TP in any patient. The results suggest that PD-ECGF/TP might give valuable information for bladder cancer management, though it may not be a good new tumor marker for bladder cancer.

Original languageEnglish
Pages (from-to)1344-1350
Number of pages7
JournalJapanese Journal of Cancer Research
Volume90
Issue number12
DOIs
Publication statusPublished - 1999 Dec

Keywords

  • Bladder transitional cell carcinoma
  • Platelet-derived endothelial cell growth factor
  • Thymidine phosphorylase

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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