Expression of platelet-derived endothelial cell growth factor and its potential role in up-regulation of angiogenesis in scarred kidneys secondary to urinary tract diseases

Ryuichiro Konda, Hiroshi Sato, Kiyohide Sakai, Makoto Sato, Seichi Orikasa, Noriko Kimura

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The mechanism of neovascularization secondary to renal interstitial fibrosis is not well understood. Platelet-derived endothelial cell growth factor (PD-ECGF) is known to promote angiogenesis. We examined the expression of PD-ECGF immunohistochemically in 9 normal kidneys and 26 scarred kidneys secondary to urinary tract diseases. To estimate up-regulation of angiogenesis, microvessels were counted by immunostaining endothelial cells for CD34. Immunostaining of PD-ECGF was observed in most Bowman's capsules, occasional tubules, and some interstitial mononuclear cells in normal kidneys. A remarkable increase of immunostained PD-ECGF was found in the tubules and interstitial mononuclear infiltrates in the scarred kidneys. The predominant cell type in the infiltrate was T cells (CD3+). The microvessel count and mean numbers of PD-ECGF+ tubular and interstitial mononuclear cells increased with increasing interstitial fibrosis. A significant correlation was noted between microvessel count and the number of PD-ECGF+ tubular cells (P = 0.0002) or PD-ECGF+ interstitial mononuclear cells (P < 0.0001). Immunostaining of endogrin, a marker of endothelial proliferation, increased in the microvessels located in the fibrotic interstitial spaces. These results suggest that angiogenesis may play a critical role in the progression of tubulointerstitial injuries and that up-regulation of PD-ECGF may contribute to neovascularization.

Original languageEnglish
Pages (from-to)1587-1597
Number of pages11
JournalAmerican Journal of Pathology
Volume155
Issue number5
DOIs
Publication statusPublished - 1999 Nov

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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