Expression of OX40 and OX40 ligand (gp34) in the normal and myasthenic thymus

J. Onodera, T. Nagata, K. Fujihara, M. Ohuchi, N. Ishii, K. Sugamura, Y. Itoyama

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Objectives - To examine the expression of OX40, an activated memory T-cell marker, and its ligand (OX40L), a set of molecules for T-cell-B-cell interaction, and other lymphocyte activation markers in the thymuses of myasthenia gravis (MG) and controls. Material and methods - We studied the expression of OX40, OX40L, IL-2Rα and HLA-DR in the thymic tissues of MG and controls using immunocytochemistry and flowcytometry. Results - In both hyperplastic thymus of MG and control thymus, OX40+ cells were scattered mainly in the medulla with much fewer OX40L+ cells being distributed in the corticomedullary junctions. IL-2Rα and HLA-DR were expressed in the medulla at higher frequencies as compared with OX40 in controls as well as MG. In contrast, the numbers of OX40+ cells around the germinal centers (GC) were significantly greater than those of control thymuses, and some mononuclear cells in GC were OX40L+. A considerable number of OX40+ cells were seen in the thymic tissues adjacent to thymomas. OX40+ cells were CD4+CD8- or CD4+CD8+ and were mostly HLA-DR-. (The coexpression of OX40 and IL-2Rα on activated CD4+ T cells was previously reported.) Conclusion - OX40, expressed in a fraction of activated CD4+ T cells, may be upregulated in thymic tissues adjacent to GC and thymoma in MG, and OX40 may interact with OX40L in GC to enhance anti-acetylcholine receptor antibody production in MG.

Original languageEnglish
Pages (from-to)236-243
Number of pages8
JournalActa Neurologica Scandinavica
Volume102
Issue number4
DOIs
Publication statusPublished - 2000

Keywords

  • Flowcytometry
  • Gp34
  • Immunocytochemistry
  • Myasthenia gravis
  • OX40
  • OX40 ligand
  • Thymus

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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