TY - JOUR
T1 - Expression of neutrophil gelatinase-associated lipocalin (NGAL) in peripheral nerve repair
AU - Aghanasir, Fatemeh
AU - Aghaei, Hassan
AU - Imani Fooladi, Abbas Ali
AU - Ebrahimi, Majid
AU - Bagherpour, Ghasem
AU - Nourani, Mohammad Reza
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor and Francis Group.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/7/3
Y1 - 2016/7/3
N2 - Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damage. Free radicals are usually scavenged and detoxied by antioxidants. In this study, we assessed the antioxidative role of the NGAL molecule in sciatic nerve repair in rats. Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the NGAL gene was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were xed in paraformaldehyde and cut in 20 micrometer slices by cryostat. Results: The expression of NGAL signicantly upregulated in day 1, 3 and week 1 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of this gene. Discussion: The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.
AB - Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damage. Free radicals are usually scavenged and detoxied by antioxidants. In this study, we assessed the antioxidative role of the NGAL molecule in sciatic nerve repair in rats. Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the NGAL gene was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were xed in paraformaldehyde and cut in 20 micrometer slices by cryostat. Results: The expression of NGAL signicantly upregulated in day 1, 3 and week 1 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of this gene. Discussion: The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.
KW - NGAL
KW - peripheral nerve
KW - repair
UR - http://www.scopus.com/inward/record.url?scp=84963859129&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963859129&partnerID=8YFLogxK
U2 - 10.3109/10799893.2015.1132238
DO - 10.3109/10799893.2015.1132238
M3 - Article
C2 - 27087673
AN - SCOPUS:84963859129
VL - 36
SP - 429
EP - 434
JO - Journal of Receptor and Signal Transduction Research
JF - Journal of Receptor and Signal Transduction Research
SN - 1079-9893
IS - 4
ER -