Expression of neutrophil gelatinase-associated lipocalin (NGAL) in peripheral nerve repair

Fatemeh Aghanasir, Hassan Aghaei, Abbas Ali Imani Fooladi, Majid Ebrahimi, Ghasem Bagherpour, Mohammad Reza Nourani

    Research output: Contribution to journalArticle

    Abstract

    Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damage. Free radicals are usually scavenged and detoxied by antioxidants. In this study, we assessed the antioxidative role of the NGAL molecule in sciatic nerve repair in rats. Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the NGAL gene was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were xed in paraformaldehyde and cut in 20 micrometer slices by cryostat. Results: The expression of NGAL signicantly upregulated in day 1, 3 and week 1 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of this gene. Discussion: The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.

    Original languageEnglish
    Pages (from-to)429-434
    Number of pages6
    JournalJournal of Receptors and Signal Transduction
    Volume36
    Issue number4
    DOIs
    Publication statusPublished - 2016 Jul 3

    Keywords

    • NGAL
    • peripheral nerve
    • repair

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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  • Cite this

    Aghanasir, F., Aghaei, H., Imani Fooladi, A. A., Ebrahimi, M., Bagherpour, G., & Nourani, M. R. (2016). Expression of neutrophil gelatinase-associated lipocalin (NGAL) in peripheral nerve repair. Journal of Receptors and Signal Transduction, 36(4), 429-434. https://doi.org/10.3109/10799893.2015.1132238