Expression of Multidrug Resistance Gene and Localization of P-Glycoprotein in Human Primary Ovarian Cancer

Shinji Arao, Hirofumi Suwa, Masaki Mandai, Hiroshi Nomura, Hiroshi Hiai, Manabu Fukumoto

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)


Resistance to chemotherapy is the major obstacle to controlling malignant tumors. To characterize multidrug resistance phenotype in human primary ovarian cancer without chemotherapy, expressions of the mdrl gene in 52 cases of ovarian cancer (44 common epithelial, 5 nonepithelial, and 3 metastatic cancers) were analyzed by polymerase chain reaction of RNA after reverse transcription. Furthermore, localization of P-glycoprotein, which is encoded by the mdrl gene, was studied immunohistochemically. Although overall expression of the mdrl gene was relatively low, its expression level was the highest in well-differentiated cancer tissues. Serous and mucinous adenocarcinomas showed higher levels of expression compared with clear cell and endometrioid carcinomas. P-glycoprotein was positive on luminal surfaces of lining cells of ovarian cancer and on those of inclusion cysts from which epithelial ovarian cancer is considered to develop. Thus, some ovarian cancer cases before chemotherapy are intrinsically multidrug resistant, which can be determined by mdrl gene expression, and this phenotype should be taken into account for effective chemotherapy of ovarian epithelial carcinomas.

Original languageEnglish
Pages (from-to)1355-1359
Number of pages5
JournalCancer Research
Issue number5
Publication statusPublished - 1994 Mar 1
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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