TY - JOUR
T1 - Expression of mRNA for matrix γ-carboxyglutamic acid protein during progression of atherosclerosis in aortae of watanabe heritable hyperlipidemic rabbits
AU - Sohma, Yoshiaki
AU - Suzuki, Takashi
AU - Sasano, Hironobu
AU - Nagura, Hiroshi
AU - Nose, Masato
AU - Yamamoto, Tokuo
PY - 1994/10
Y1 - 1994/10
N2 - In an effort to characterize mRNAs that are highly expressed during atherosclerosis, we employed differential hybridization screening of a cDNA library constructed from total RNA derived from the aorta of Watanabe heritable hyperlipidemic (WHHL) rabbits. Characterizing the cDNAs for mRNAs that are present in large amounts in WHHL rabbit aortae, we identified a positive clone encoding matrix γ-carboxyglutamic acid protein (MGP). The primary structure of rabbit MGP was deduced from nucleotide sequence analysis of the cDNA. Northern blot analysis of total RNA prepared from aortae of WHHL and normal rabbits of various ages indicated that the expression of MGP mRNA increased in proportion to the progression of atherosclerosis in WHHL rabbits. Analysis of MGP mRNA by in situ hybridization revealed that a significant amount of MGP mRNA is accumulated in atherosclerotic lesions of WHHL rabbits, suggesting that the expression of MGP mRNA is correlated with the progression of atherosclerosis.
AB - In an effort to characterize mRNAs that are highly expressed during atherosclerosis, we employed differential hybridization screening of a cDNA library constructed from total RNA derived from the aorta of Watanabe heritable hyperlipidemic (WHHL) rabbits. Characterizing the cDNAs for mRNAs that are present in large amounts in WHHL rabbit aortae, we identified a positive clone encoding matrix γ-carboxyglutamic acid protein (MGP). The primary structure of rabbit MGP was deduced from nucleotide sequence analysis of the cDNA. Northern blot analysis of total RNA prepared from aortae of WHHL and normal rabbits of various ages indicated that the expression of MGP mRNA increased in proportion to the progression of atherosclerosis in WHHL rabbits. Analysis of MGP mRNA by in situ hybridization revealed that a significant amount of MGP mRNA is accumulated in atherosclerotic lesions of WHHL rabbits, suggesting that the expression of MGP mRNA is correlated with the progression of atherosclerosis.
KW - Atherosclerosis
KW - Differential hybridization
KW - Matrix γ-carboxyglutamic acid protein
KW - Watanabe heritable hyperlipidemic rabbit.
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U2 - 10.1093/oxfordjournals.jbchem.a124591
DO - 10.1093/oxfordjournals.jbchem.a124591
M3 - Article
C2 - 7883748
AN - SCOPUS:0028097234
VL - 116
SP - 747
EP - 751
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 4
ER -