TY - JOUR
T1 - Expression of MMP-8 and MMP-13 mRNAs in Rat Periodontium during Tooth Eruption
AU - Tsubota, M.
AU - Sasano, Y.
AU - Takahashi, I.
AU - Kagayama, M.
AU - Shimauchi, Hidetoshi
N1 - Funding Information:
We thank Dr. Hiroshi Horiuchi, Professor Emeritus at Tohoku University, for his constructive suggestions to this study. We also thank Mr. Masami Eguchi and Mr. Yasuto Mikami, Division of Oral Molecular Biology, Tohoku University Graduate School of Dentistry, for their excellent assistance in this study. This work was supported in part by a grant-in-aid (13671893) from the Ministry of Education, Science, Sports and Culture of Japan.
Publisher Copyright:
© 2002, International and American Associations for Dental Research.
PY - 2002/10
Y1 - 2002/10
N2 - The present study was designed to investigate mRNA expression of matrix metalloproteinase-8 (MMP-8) and MMP-13 in forming periodontium during tooth eruption in the rat. RT-PCR for the decalcified paraffin sections indicated expression of MMP-8 and MMP-13 in the periodontal tissues. In situ hydridization demonstrated expression of MMP-8 in osteoblasts, osteocytes, periodontal ligament cells, cementoblasts, and cementocytes along with collagen types I and III. In contrast, transcripts of MMP-13 were confined to a small population of osteoblasts and osteocytes in alveolar bone. The results suggested that MMP-8 may be involved in remodeling the periodontium during tooth eruption, and its expression may be coordinated with that of collagen types I and III, whereas the participation of MMP-13 may be rather limited.
AB - The present study was designed to investigate mRNA expression of matrix metalloproteinase-8 (MMP-8) and MMP-13 in forming periodontium during tooth eruption in the rat. RT-PCR for the decalcified paraffin sections indicated expression of MMP-8 and MMP-13 in the periodontal tissues. In situ hydridization demonstrated expression of MMP-8 in osteoblasts, osteocytes, periodontal ligament cells, cementoblasts, and cementocytes along with collagen types I and III. In contrast, transcripts of MMP-13 were confined to a small population of osteoblasts and osteocytes in alveolar bone. The results suggested that MMP-8 may be involved in remodeling the periodontium during tooth eruption, and its expression may be coordinated with that of collagen types I and III, whereas the participation of MMP-13 may be rather limited.
KW - RT-PCR
KW - collagen
KW - collagenase
KW - in situ hybridization
KW - periodontal ligament
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U2 - 10.1177/154405910208101004
DO - 10.1177/154405910208101004
M3 - Article
C2 - 12351664
AN - SCOPUS:0036802851
VL - 81
SP - 673
EP - 678
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 10
ER -