TY - JOUR
T1 - Expression of MIP-3α/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma
AU - Abiko, Yoshihiro
AU - Nishimura, Michiko
AU - Kusano, Kaoru
AU - Nakashima, Keisuke
AU - Okumura, Kazuhiko
AU - Arakawa, Toshiya
AU - Takuma, Taishin
AU - Mizoguchi, Itaru
AU - Kaku, Tohru
PY - 2003/2/1
Y1 - 2003/2/1
N2 - We have examined the expression of MIP-3α/CCL20 in oral squamous cell carcinoma (SCC) in vivo and in vitro. In addition, we have investigated whether the expression of MIP-3α/CCL20 is regulated by bacterial infection and inflammatory cytokines. In order to determine the mRNA level of MIP-3α, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed with LightCycler™ using the double-stranded DNA dye, SYBR Green I. Oral epithelial cells and six SCC cell lines (SCC-9, SAS, BSC-OF, HSC-4, HSC, Ca9-22) were found to express MIP-3α mRNA. The expression of MIP-3α was upregulated by infection with Actinobacillus actinomycetemcomitans and by stimulation with lipopolysaccharide and TNF-α. By in situ hybridization, the detectable MIP-3α expression in SCC was localized primarily at the epithelial pearls corresponding to the spinous layer. These results suggest that MIP-3α contributes to the oral immunoresponse to bacterial infection, and may be involved in the growth of SCC.
AB - We have examined the expression of MIP-3α/CCL20 in oral squamous cell carcinoma (SCC) in vivo and in vitro. In addition, we have investigated whether the expression of MIP-3α/CCL20 is regulated by bacterial infection and inflammatory cytokines. In order to determine the mRNA level of MIP-3α, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed with LightCycler™ using the double-stranded DNA dye, SYBR Green I. Oral epithelial cells and six SCC cell lines (SCC-9, SAS, BSC-OF, HSC-4, HSC, Ca9-22) were found to express MIP-3α mRNA. The expression of MIP-3α was upregulated by infection with Actinobacillus actinomycetemcomitans and by stimulation with lipopolysaccharide and TNF-α. By in situ hybridization, the detectable MIP-3α expression in SCC was localized primarily at the epithelial pearls corresponding to the spinous layer. These results suggest that MIP-3α contributes to the oral immunoresponse to bacterial infection, and may be involved in the growth of SCC.
KW - Bacterial infection
KW - Cytokine
KW - MIP-3α/LARC/EXODUS/CCL20
KW - Quantitative RT-PCR
KW - Squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=0037309763&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037309763&partnerID=8YFLogxK
U2 - 10.1016/S0003-9969(02)00167-X
DO - 10.1016/S0003-9969(02)00167-X
M3 - Article
C2 - 12642237
AN - SCOPUS:0037309763
VL - 48
SP - 171
EP - 175
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 2
ER -