Expression of MIP-3α/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma

Yoshihiro Abiko, Michiko Nishimura, Kaoru Kusano, Keisuke Nakashima, Kazuhiko Okumura, Toshiya Arakawa, Taishin Takuma, Itaru Mizoguchi, Tohru Kaku

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

We have examined the expression of MIP-3α/CCL20 in oral squamous cell carcinoma (SCC) in vivo and in vitro. In addition, we have investigated whether the expression of MIP-3α/CCL20 is regulated by bacterial infection and inflammatory cytokines. In order to determine the mRNA level of MIP-3α, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed with LightCycler™ using the double-stranded DNA dye, SYBR Green I. Oral epithelial cells and six SCC cell lines (SCC-9, SAS, BSC-OF, HSC-4, HSC, Ca9-22) were found to express MIP-3α mRNA. The expression of MIP-3α was upregulated by infection with Actinobacillus actinomycetemcomitans and by stimulation with lipopolysaccharide and TNF-α. By in situ hybridization, the detectable MIP-3α expression in SCC was localized primarily at the epithelial pearls corresponding to the spinous layer. These results suggest that MIP-3α contributes to the oral immunoresponse to bacterial infection, and may be involved in the growth of SCC.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalArchives of Oral Biology
Volume48
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1
Externally publishedYes

Keywords

  • Bacterial infection
  • Cytokine
  • MIP-3α/LARC/EXODUS/CCL20
  • Quantitative RT-PCR
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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