Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles

Yasuyuki Sasano, Jing Xu Zhu, Shinji Kamakura, Shinichiro Kusunoki, Itaru Mizoguchi, Manabu Kagayama

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

It is not known how bone proteins appear in the matrix before and after calcification during embryonic osteogenesis. The present study was designed to investigate expressions of the five major bone extracellular matrix proteins - i.e. type I collagen, osteonectin, osteopontin, bone sialoprotein and osteocalcin - during osteogenesis in rat embryonic mandibles immunohistochemically, and their involvement in calcification demonstrated by von Kossa staining. Wistar rat embryos 14 to 18 days post coitum were used. Osteogenesis was not seen in 14-day rat embryonic mandibles. Type I collagen was localized in the uncalcifed bone matrix in 15-day mandibles, where no other bone proteins showed immunoreactivity. Osteonectin, osteopontin, bone sialoprotein and osteocalcin appeared almost simultaneously in the calcified bone matrix of 16-day mandibles and accumulated continuously in 18-day mandibles. The present study suggested that type I collagen constitutes the basic framework of the bone matrix upon which the noncollagenous proteins are oriented to lead to calcification, whereas the noncollagenous proteins are deposited simultaneously by osteoblasts and are involved in calcification cooperatively.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalAnatomy and Embryology
Volume202
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Bone
  • Calcification
  • Immunohistochemistry
  • Noncollagenous proteins
  • Type I collagen

ASJC Scopus subject areas

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

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