Expression of liver X receptor α in rat fetal tissues at different developmental stages

Azusa Sakamoto, Takashi Kawasaki, Toshihiro Kazawa, Riuko Ohashi, Shuying Jiang, Takashi Maejima, Toshiya Tanaka, Hiroko Iwanari, Takao Hamakubo, Juro Sakai, Tatsuhiko Kodama, Makoto Naito

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The liver X receptor (LXR) is a nuclear receptor that acts as a sterol sensor and metabolic regulator of cholesterol and lipid homeostasis. Using a novel LXRα-specific antibody for immunohistochemistry, we evaluated cellular expression of LXRα in fetal rat tissues. In the fetal liver, LXRα-positive macrophages appeared at 12 days and their number peaked at 18 days of gestation. In contrast, hepatocytes expressed LXRα during the later stage of gestation, suggesting the functional development of the liver during ontogeny. Later, macrophages in spleen and thymus expressed LXRα, and some mononuclear cells in the vascular lumen compatible to primitive/fetal macrophages in the fetal circulation were found to express LXRα. In vitro, rat monocytes did not express LXRα, but monocyte-derived macrophages cultured in the presence of macrophage-colony stimulating factor revealed the distinct expression of LXRα in nucleoli. These findings suggest that LXRα plays a role in the differentiation of fetal macrophages, particularly hepatic macrophages, in rat development.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalJournal of Histochemistry and Cytochemistry
Volume55
Issue number6
DOIs
Publication statusPublished - 2007 Jun
Externally publishedYes

Keywords

  • Development
  • LXRα
  • Liver
  • Macrophage
  • Nucleoli
  • Rat

ASJC Scopus subject areas

  • Anatomy
  • Histology

Fingerprint Dive into the research topics of 'Expression of liver X receptor α in rat fetal tissues at different developmental stages'. Together they form a unique fingerprint.

Cite this