Expression of liver-type fatty acid-binding protein in murine lung and its release into serum upon challenge of lung with lipopolysaccharide

Pattira Piumngam, Christian Schachtrup, Yuji Owada, Hisatake Kondo, Chamras Promptmas, Friedrich Spener

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fatty acid-binding proteins (FABP) in alveolar type II (TII) cells are required for surfactant synthesis and regulation. Beyond expression of heart-type (H-) and epidermal-type (E-) FABP in TII cells from mouse lung, we present the first evidence of the expression of liver-type (L-) FABP, by quantitative PCR and immunofluorescent confocal laser microscopy. Further, by making use of an acute mouse lung injury model, we examine whether these lipid-binding proteins are released into the bronchoalveolar fluid (BALF) and into the circulation upon challenge of the lung with lipopolysaccharide. Applying FABP-specific ELISAs, we found that neither H- nor E-FABP can be detected in BALF and serum above background levels, up to 24 h after insult. In contrast, L-FABP was detected in the BALF pellet, consisting of polymorphonuclear cells and alveolar macrophages, and in serum. A significant decrease in L-FABP levels in the BALF pellet was associated with a significant increase in serum levels 6 h post insult. As contributions of L-FABP from other organs were excluded, this protein could be used as a marker for acute lung injury.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalEuropean Journal of Lipid Science and Technology
Volume107
Issue number3
DOIs
Publication statusPublished - 2005

Keywords

  • Acute lung damage
  • Alveolar type II cell
  • Lipopolysaccaride
  • Liver-type fatty acid-binding protein
  • Serum

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Chemistry(all)
  • Industrial and Manufacturing Engineering

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