Expression of Ins1 and Ins2 genes in mouse fetal liver

Shoko Murakami-Kawaguchi, Shin Takasawa, Tohru Onogawa, Koji Nata, Asako Itaya-Hironaka, Sumiyo Sakuramoto-Tsuchida, Akiyo Yamauchi, Hiroyo Ota, Maiko Takeda, Masato Kato, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A possible cure for diabetes is explored by using non-pancreatic cells such as fetal hepatocytes. The expression of insulin and transcription factors for insulin is investigated in mouse fetal liver. We detected mRNAs for insulin I (Ins1) and insulin II (Ins2) and proinsulin- and mature insulin-positive cells in mouse fetal liver by reverse transcription plus the polymerase chain reaction and immunohistochemistry. Glucagon, somatostatin and pancreatic polypeptide were not expressed throughout development. Mouse Ins2 and Ins1 promoters were transiently activated in mouse fetal hepatocytes of embryonic days 13.5 and 16.5, respectively. Pancreatic and duodenal homeobox 1 (Pdx1) mRNA was not expressed during development of the liver. In contrast, mRNAs and proteins of neurogenic differentiation (NeuroD)/β cell E-box transactivator 2 (Beta2) and v-maf musculoaponeurotic fibrosarcoma oncogene homolog (MafA) were almost simultaneously expressed with insulin genes in the liver. Ins2 and Ins1 promoters were activated in hepatoma cells by the transfection of the expression vector for NeuroD/Beta2 alone and by the combination of NeuroD/Beta2 and MafA, respectively. These results indicate that the expression of NeuroD/Beta2 and MafA is linked temporally with the transcription of Ins2 and Ins1 genes in mouse fetal liver and suggest the potential usage of fetal hepatocytes to make insulin-producing β cells by introducing transcription factors.

Original languageEnglish
Pages (from-to)303-314
Number of pages12
JournalCell and Tissue Research
Volume355
Issue number2
DOIs
Publication statusPublished - 2014 Feb

Keywords

  • Fetal liver
  • Human
  • Insulin genes
  • MafA
  • Mouse
  • NeuroD/Beta2
  • Rat
  • Transcription

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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