Expression of inflammatory-related factors in porcine anterior pituitary-derived cell line

Yasuhiro Nagai, Hideki Ogasawara, Yoshikazu Taketa, Hisashi Aso, Takashi Kanaya, Masato Miyake, Kouichi Watanabe, Shyuichi Ohwada, Yoshihiro Muneta, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Recent studies have shown that undifferentiated stem cells act as immunomodulators. To investigate the immunomodulatory function of the progenitor cells of the anterior pituitary gland, we attempted to establish a stem/progenitor cell line from the porcine anterior pituitary gland, and to detail its inflammatory cytokine expression. A cloned cell line from the porcine anterior pituitary gland was established and was designated as the porcine anterior pituitary-derived cell line (PAPC). PAPC expressed the mRNA of Nanog and Oct-4, and showed positive immunoreactivity for β-catenin and Hes1 in its nucleus. PAPC grew stably by repeated passage and rapidly in the EGF and bFGF containing medium. RT-PCR showed that PAPC expressed mRNA of IL-1α, IL-6, IL-12, IL-15, IL-18 and TLR4. PAPC expressed S100α and IL-18 protein, which was localized in the marginal epithelial cells of Rathke's pouch. These results suggest that PAPC is a stem/progenitor cell and may regulate anterior pituitary cell function through an immuno-endocrine pathway.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume124
Issue number3-4
DOIs
Publication statusPublished - 2008 Aug 15

Keywords

  • Immuno-endocrine
  • Inflammatory cytokines
  • PAPC
  • Porcine anterior pituitary gland
  • Stem/progenitor cells

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Expression of inflammatory-related factors in porcine anterior pituitary-derived cell line'. Together they form a unique fingerprint.

Cite this