TY - JOUR
T1 - Expression of inflammatory-related factors in porcine anterior pituitary-derived cell line
AU - Nagai, Yasuhiro
AU - Ogasawara, Hideki
AU - Taketa, Yoshikazu
AU - Aso, Hisashi
AU - Kanaya, Takashi
AU - Miyake, Masato
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Muneta, Yoshihiro
AU - Yamaguchi, Takahiro
N1 - Funding Information:
This work was supported by a Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2008/8/15
Y1 - 2008/8/15
N2 - Recent studies have shown that undifferentiated stem cells act as immunomodulators. To investigate the immunomodulatory function of the progenitor cells of the anterior pituitary gland, we attempted to establish a stem/progenitor cell line from the porcine anterior pituitary gland, and to detail its inflammatory cytokine expression. A cloned cell line from the porcine anterior pituitary gland was established and was designated as the porcine anterior pituitary-derived cell line (PAPC). PAPC expressed the mRNA of Nanog and Oct-4, and showed positive immunoreactivity for β-catenin and Hes1 in its nucleus. PAPC grew stably by repeated passage and rapidly in the EGF and bFGF containing medium. RT-PCR showed that PAPC expressed mRNA of IL-1α, IL-6, IL-12, IL-15, IL-18 and TLR4. PAPC expressed S100α and IL-18 protein, which was localized in the marginal epithelial cells of Rathke's pouch. These results suggest that PAPC is a stem/progenitor cell and may regulate anterior pituitary cell function through an immuno-endocrine pathway.
AB - Recent studies have shown that undifferentiated stem cells act as immunomodulators. To investigate the immunomodulatory function of the progenitor cells of the anterior pituitary gland, we attempted to establish a stem/progenitor cell line from the porcine anterior pituitary gland, and to detail its inflammatory cytokine expression. A cloned cell line from the porcine anterior pituitary gland was established and was designated as the porcine anterior pituitary-derived cell line (PAPC). PAPC expressed the mRNA of Nanog and Oct-4, and showed positive immunoreactivity for β-catenin and Hes1 in its nucleus. PAPC grew stably by repeated passage and rapidly in the EGF and bFGF containing medium. RT-PCR showed that PAPC expressed mRNA of IL-1α, IL-6, IL-12, IL-15, IL-18 and TLR4. PAPC expressed S100α and IL-18 protein, which was localized in the marginal epithelial cells of Rathke's pouch. These results suggest that PAPC is a stem/progenitor cell and may regulate anterior pituitary cell function through an immuno-endocrine pathway.
KW - Immuno-endocrine
KW - Inflammatory cytokines
KW - PAPC
KW - Porcine anterior pituitary gland
KW - Stem/progenitor cells
UR - http://www.scopus.com/inward/record.url?scp=47149098814&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47149098814&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2008.03.007
DO - 10.1016/j.vetimm.2008.03.007
M3 - Article
C2 - 18440649
AN - SCOPUS:47149098814
VL - 124
SP - 201
EP - 208
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 3-4
ER -