TY - JOUR
T1 - Expression of IL-2 receptor β and γ chains by human gingival fibroblasts and up-regulation of adhesion to neutrophils in response to IL-2
AU - Ozawa, Akiko
AU - Tada, Hiroyuki
AU - Tamai, Riyoko
AU - Uehara, Akiko
AU - Watanabe, Kouichi
AU - Yamaguchi, Takahiro
AU - Shimauchi, Hidetoshi
AU - Takada, Haruhiko
AU - Sugawara, Shunji
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/9
Y1 - 2003/9
N2 - To investigate the role of human gingival fibroblasts (HGF), the major constituents of gingival tissue in periodontal inflammatory disease, the expression of interleukin-2 receptor (IL-2R) α, β, and γ chains was examined. Immunohistochemistry showed a pronounced accumulation of CD8+ T cells in the inflamed lamina propria of gingival tissue from patients with adult periodontitis. HGF express IL-2Rβ and IL-2Rγ at mRNA and protein levels, but the expression of IL-2Rα could not be detected, as assessed by reverse transcriptase-polymerase chain reaction and flow cytometry. IL-2Rβ, and -γ expressed on HGF were functionally active, as addition of neutralizing anti-IL-2Rβ and -γ antibodies caused inhibition of the IL-2-induced production of monocyte chemoattractant protein-1 (MCP-1), and addition of IL-2 induced phosphorylation of Janus tyrosine kinase 3, which is critical in signaling through IL-2Rγ in HGF. The IL-2-induced MCP-1 production was significantly inhibited by pretreatment with neutralizing antibody to IL-15. Addition of IL-2 also induced a marked up-regulation of the expression of intercellular adhesion molecule-1 (ICAM-1) on the surface of HGF, which in turn, significantly augmented the adhesion of human neutrophils, which were inhibited by an anti-ICAM-1 antibody. These results suggest that HGF express functional IL-2Rβγ, respond to IL-2 from infiltrated T cells, and actively participate in the inflammatory process in the periodontal region and that IL-15 produced by HGF sustains IL-2-mediated signaling in HGF.
AB - To investigate the role of human gingival fibroblasts (HGF), the major constituents of gingival tissue in periodontal inflammatory disease, the expression of interleukin-2 receptor (IL-2R) α, β, and γ chains was examined. Immunohistochemistry showed a pronounced accumulation of CD8+ T cells in the inflamed lamina propria of gingival tissue from patients with adult periodontitis. HGF express IL-2Rβ and IL-2Rγ at mRNA and protein levels, but the expression of IL-2Rα could not be detected, as assessed by reverse transcriptase-polymerase chain reaction and flow cytometry. IL-2Rβ, and -γ expressed on HGF were functionally active, as addition of neutralizing anti-IL-2Rβ and -γ antibodies caused inhibition of the IL-2-induced production of monocyte chemoattractant protein-1 (MCP-1), and addition of IL-2 induced phosphorylation of Janus tyrosine kinase 3, which is critical in signaling through IL-2Rγ in HGF. The IL-2-induced MCP-1 production was significantly inhibited by pretreatment with neutralizing antibody to IL-15. Addition of IL-2 also induced a marked up-regulation of the expression of intercellular adhesion molecule-1 (ICAM-1) on the surface of HGF, which in turn, significantly augmented the adhesion of human neutrophils, which were inhibited by an anti-ICAM-1 antibody. These results suggest that HGF express functional IL-2Rβγ, respond to IL-2 from infiltrated T cells, and actively participate in the inflammatory process in the periodontal region and that IL-15 produced by HGF sustains IL-2-mediated signaling in HGF.
KW - Adhesion molecule
KW - Chemokine
KW - Cytokine receptor
KW - Gingival tissue
KW - Periodontitis
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U2 - 10.1189/jlb.0103044
DO - 10.1189/jlb.0103044
M3 - Article
C2 - 12949238
AN - SCOPUS:0142029485
VL - 74
SP - 352
EP - 359
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 3
ER -