TY - JOUR
T1 - Expression of home oxygenase and inducible nitric oxide synthase mRNA in human brain tumors
AU - Hara, Eishi
AU - Takahashi, Kazuhiro
AU - Tominaga, Teiji
AU - Kumabe, Toshihiro
AU - Kayama, Takamasa
AU - Suzuki, Hiroyuki
AU - Fujita, Hiroyoshi
AU - Yoshimoto, Takashi
AU - Shirato, Kunio
AU - Shibahara, Shigeki
N1 - Funding Information:
We thank Dr. Kaoru Gotoh for helpful discussion. This study was supported in part by Grants-in Aid for Developmental Scientific Research (to H.F. and to S.S.) and for Scientific Research on Priority Areas (to S.S.) from the Ministry of Education, Science, and Culture of Japan.
PY - 1996/7/5
Y1 - 1996/7/5
N2 - Heme oxygenase-1, a key enzyme in heme catabolism, and inducible nitric oxide synthase (iNOS) are responsible for production of carbon monoxide and nitric oxide (NO), respectively. Expression of each enzyme has been shown to be modulated by heme and NO, raising a possibility for the coordinated regulation of the two enzymes. We therefore analyzed the expression levels of both mRNA in humans using brain tumors. Either heme oxygenase-1 or iNOS mRNA was expressed at higher levels in brain tumors compared to the brain tissue, but their expression levels were not apparently correlated. In the brain tumor cell lines, treatment with cytokines increased the expression of iNOS mRNA but not heme oxygenase-1 mRNA, whereas treatment with an NO donor increased the expression of heme oxygenase-1 mRNA but not iNOS mRNA. These results suggest the separate regulation of expression of both enzyme mRNA in humans.
AB - Heme oxygenase-1, a key enzyme in heme catabolism, and inducible nitric oxide synthase (iNOS) are responsible for production of carbon monoxide and nitric oxide (NO), respectively. Expression of each enzyme has been shown to be modulated by heme and NO, raising a possibility for the coordinated regulation of the two enzymes. We therefore analyzed the expression levels of both mRNA in humans using brain tumors. Either heme oxygenase-1 or iNOS mRNA was expressed at higher levels in brain tumors compared to the brain tissue, but their expression levels were not apparently correlated. In the brain tumor cell lines, treatment with cytokines increased the expression of iNOS mRNA but not heme oxygenase-1 mRNA, whereas treatment with an NO donor increased the expression of heme oxygenase-1 mRNA but not iNOS mRNA. These results suggest the separate regulation of expression of both enzyme mRNA in humans.
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U2 - 10.1006/bbrc.1996.0999
DO - 10.1006/bbrc.1996.0999
M3 - Article
C2 - 8694803
AN - SCOPUS:0030570270
VL - 224
SP - 153
EP - 158
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -