Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells

Norihito Hayatsu, Satoshi Ogasawara, Mika Kato Kaneko, Yukinari Kato, Hisashi Narimatsu

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalBiochemical and biophysical research communications
Volume368
Issue number2
DOIs
Publication statusPublished - 2008 Apr 4
Externally publishedYes

Keywords

  • Glioblastoma
  • Glycogene
  • Keratan sulfate
  • Proteoglycan

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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