Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris

Takashi Nakamura, Marcel Zámocký, Zbyněk Zdráhal, Radka Chaloupková, Marta Monincová, Zbyněk Prokop, Yuji Nagata, Jiří Damborský

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Heterologous expression of the bacterial enzyme haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 in methylotrophic yeast Pichia pastoris is reported. The haloalkane dehalogenase gene linB was subcloned into the pPICZαA vector and integrated into the genome of P. pastoris. The recombinant LinB secreted from the yeast was purified to homogeneity and biochemically characterized. The deglycosylation experiment and mass spectrometry measurements showed that the recombinant LinB expressed in P. pastoris is glycosylated with a 2.8 kDa size of high mannose core. The specific activity of the glycosylated LinB was 15.6 ± 3.7 μmol/min/mg of protein with 1,2-dibromoethane and 1.86 ± 0.36 μmol/min/mg of protein with 1-chlorobutane. Activity and solution structure of the protein produced in P. pastoris is comparable with that of recombinant LinB expressed in Escherichia coli. The melting temperature determined by the circular dichroism (41.7 ± 0.3°C for LinB expressed in P. pastoris and 41.8 ± 0.3°C expressed in E. coli) and thermal stability measured by specific activity to 1-chlorobutane were also similar for two enzymes. Our results show that LinB can be extracellularly expressed in eukaryotic cell and glycosylation had no effect on activity, protein fold and thermal stability of LinB.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalProtein Expression and Purification
Volume46
Issue number1
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Catalytic activity
  • Dehalogenation
  • Glycosylation
  • Heterologous expression
  • Pichia pastoris
  • Thermal stability

ASJC Scopus subject areas

  • Biotechnology

Fingerprint Dive into the research topics of 'Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris'. Together they form a unique fingerprint.

  • Cite this

    Nakamura, T., Zámocký, M., Zdráhal, Z., Chaloupková, R., Monincová, M., Prokop, Z., Nagata, Y., & Damborský, J. (2006). Expression of glycosylated haloalkane dehalogenase LinB in Pichia pastoris. Protein Expression and Purification, 46(1), 85-91. https://doi.org/10.1016/j.pep.2005.08.022