Expression of GLUT4 glucose transporter protein in adipose tissue and skeletal muscle from streptozotocin-induced diabetic pregnant rats

K. Yamada, K. Yamakawa, Y. Terada, K. Kawaguchi, A. Sugaya, Takashi Sugiyama, N. Toyoda

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Depletion of GLUT4, the primary glucose transporter protein in adipose tissue and skeletal muscle, is reported to contribute to insulin resistance in pregnancy or diabetes. To examine this phenomenon, the expression of GLUT4 protein was assessed by Western blotting in streptozotocin-induced diabetic pregnant rats. In adipose tissue, relative to control, it was decreased by 30% in the normal pregnant group (p<0.001), by 37% in the diabetic nonpregnant group (p<0.01) and by 65% in the diabetic pregnant group (p < 0.001). On the other hand, no significant variation was evident among the groups in skeletal muscle. To assess the mechanisms responsible for depletion of GLUT4 protein in adipose tissue, we quantitated levels of GLUT4 mRNA with a RNase protection assay. It was decreased by 44% in the normal pregnant group (p < 0.05) and by 55% in the diabetic pregnant group (p < 0.05), but not altered in the diabetic nonpregnant group. These results suggest that the depletion of GLUT4 protein in adipose tissue is a factor contributing to insulin resistance in pregnancy or diabetes, especially when the two states exist in combination.

Original languageEnglish
Pages (from-to)508-513
Number of pages6
JournalHormone and Metabolic Research
Volume31
Issue number9
DOIs
Publication statusPublished - 1999

Keywords

  • Adipose Tissue
  • GLUT4
  • Pregnancy
  • Rat
  • Skeletal Muscle
  • Streptozotocin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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