Expression of gene for Dioscorea batatas tuber lectin 1 in transgenic tobacco confers resistance to green-peach aphid

Tetsuya Kato, Masatoshi Hori, Tomohisa Ogawa, Koji Muramoto, Kinya Toriyama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Dioscorea batatas tuber lectin 1 (DB1) is a storage protein isolated from yam tuber, and is shown to be a mannose-binding lectin. It has 58% amino-acid identity to insecticidal snowdrop bulb lectin GNA. In this study, we demonstrated that ≥ 1 mg ml-1 DB1 in an artificial diet significantly decreased the survival and fecundity of green peach aphid, Myzus persicaeca. We produced transgenic tobacco plants expressing cDNA of DB1 under the control of Cauliflower mosaic virus 35S promoter (35S-DB1) or phloem-specific promoter of rice sucrose synthase-1 gene (RSs1-DB1), and evaluate the degree of aphid resistance in whole plant bioassays. The number of survival aphids was reduced to 60% in transgenic lines with 35S-DB1 and RSs1-DB1, which accumulated DB1 at a level of 1.8% and 0.25%, respectively, of total soluble protein. Our results indicate that DB1 can be used to enhance resistance to sap-sucking insects in transgenic crops.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalPlant Biotechnology
Volume27
Issue number2
DOIs
Publication statusPublished - 2010 Jan 1

Keywords

  • Aphid
  • Dioscorea batatas tuber lectin 1
  • Insect resistance
  • Transgenic tobacco

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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