Expression of fugu TIMP-3 and -4 genes in adult tissues and embryos

Hiroshi Tsukamoto, Yoshihiro Yokoyama, Tohru Suzuki, Shoshi Mizuta, Reiji Yoshinaka

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    The tissue inhibitors of metalloproteinases (TIMPs) are involved in various processes of extra-cellular matrix (ECM) metabolism by inhibiting matrix metalloproteinases (MMPs). However, the fundamental information for these genes is little known in fish. Previously, we report cDNA cloning and gene expressions of two fugu (Takifugu rubripes) TIMP-2s. Here, we cloned cDNA of fugu TIMP-3 and performed an expression analysis of TIMP-3 and -4 mRNA in fugu adult tissues using a quantitative real-time PCR. The expression level of TIMP-3 mRNA was constitutive in all tissues, while TIMP-4 was significantly higher in the brain (P = 0.05). Further, we performed a whole mount in situ hybridization in fugu embryos at different stages. In early stages, TIMP-3 mRNA was abundant in the somites and the caudal end of the notochord. At hatching larvae, the TIMP-3 mRNA was abundant in the pectoral fin, dorsal and ventral fin fold along the entire antero-posterior axis. TIMP-3 may be involved in axis elongation and somitogenesis. TIMP-4 mRNA was expressed in the tail bud, at the midbrain-hindbrain boundary and in the diencephalon from 72 to 104 hpf. This indicates TIMP-4 is highly expressed in the brain matrix in vivo.

    Original languageEnglish
    Pages (from-to)395-403
    Number of pages9
    JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
    Volume144
    Issue number3
    DOIs
    Publication statusPublished - 2006 Jul

    Keywords

    • Embryo
    • Fish
    • Quantitative real-time PCR
    • Rapid amplification of cDNA ends (RACE)
    • TIMP-3
    • TIMP-4
    • Takifugu rubripes
    • Teleost
    • Whole mount in situ hybridization

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Molecular Biology

    Fingerprint

    Dive into the research topics of 'Expression of fugu TIMP-3 and -4 genes in adult tissues and embryos'. Together they form a unique fingerprint.

    Cite this