Expression of fugu TIMP-3 and -4 genes in adult tissues and embryos

Hiroshi Tsukamoto, Yoshihiro Yokoyama, Tohru Suzuki, Shoshi Mizuta, Reiji Yoshinaka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The tissue inhibitors of metalloproteinases (TIMPs) are involved in various processes of extra-cellular matrix (ECM) metabolism by inhibiting matrix metalloproteinases (MMPs). However, the fundamental information for these genes is little known in fish. Previously, we report cDNA cloning and gene expressions of two fugu (Takifugu rubripes) TIMP-2s. Here, we cloned cDNA of fugu TIMP-3 and performed an expression analysis of TIMP-3 and -4 mRNA in fugu adult tissues using a quantitative real-time PCR. The expression level of TIMP-3 mRNA was constitutive in all tissues, while TIMP-4 was significantly higher in the brain (P = 0.05). Further, we performed a whole mount in situ hybridization in fugu embryos at different stages. In early stages, TIMP-3 mRNA was abundant in the somites and the caudal end of the notochord. At hatching larvae, the TIMP-3 mRNA was abundant in the pectoral fin, dorsal and ventral fin fold along the entire antero-posterior axis. TIMP-3 may be involved in axis elongation and somitogenesis. TIMP-4 mRNA was expressed in the tail bud, at the midbrain-hindbrain boundary and in the diencephalon from 72 to 104 hpf. This indicates TIMP-4 is highly expressed in the brain matrix in vivo.

Original languageEnglish
Pages (from-to)395-403
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume144
Issue number3
DOIs
Publication statusPublished - 2006 Jul

Keywords

  • Embryo
  • Fish
  • Quantitative real-time PCR
  • Rapid amplification of cDNA ends (RACE)
  • TIMP-3
  • TIMP-4
  • Takifugu rubripes
  • Teleost
  • Whole mount in situ hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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