Expression of five steroidogenic genes including aromatase gene at early developmental stages of chicken male and female embryos

Osamu Nomura, Osamu Nakabayashi, Katsuhiko Nishimori, Hiroshi Yasue, Shigeki Mizuno

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

In the course of avian embryo development, estrogen has been indicated to play a key role in gonadal differentiation by the inhibition of aromatase (P-450arom) that synthesizes estrogen from androgen. Biosynthesis of estrogen requires not only P-450arom but also other enzymes for a steroidogenic pathway. To elucidate gonadal differentiation, the steroidogenic pathway should be studied comprehensively in the early developmental stages including that of sex differentiation. Therefore, in the present study, the expressions of the steroidogenic genes, P-450scc, 3β-HSD, P-450c17, 17β-HSD and P- 450arom, were measured at the developmental stages (days 2-9 of incubation) of chicken embryos by quantitative RT-PCR. Transcripts for all the genes studied, except for P-450arom were detected in all the developmental stages examined, indicating that mRNAs for the steroidogenic enzymes required to convert cholesterol to androgens are present in the avian embryo before gonadal differentiation. In contrast, P-450arom mRNA was detected in female embryos during days 5-9 of incubation but not in male embryos throughout incubation. The onset of P-450arom gene expression at day 5 coincides with the stage of gonadal differentiation, corroborating the role of estrogen in the process of gonadal differentiation in chicken.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume71
Issue number3-4
DOIs
Publication statusPublished - 1999 Dec 15

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of five steroidogenic genes including aromatase gene at early developmental stages of chicken male and female embryos'. Together they form a unique fingerprint.

Cite this