Expression of cytochrome P-450d by Saccharomyces cerevisiae

Toru Shimizu, Kazuhiro Sogawa, Yoshiaki Fujii-Kuriyama, Masae Takahashi, Yoshiro Ogoma, Masahiro Hatano

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalFEBS Letters
Issue number2
Publication statusPublished - 1986 Oct 27


  • (Yeast)
  • Cytochrome P-450
  • Expression
  • cDNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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