Expression of cytochrome P-450d by Saccharomyces cerevisiae

Toru Shimizu, Kazuhiro Sogawa, Yoshiaki Fujii-Kuriyama, Masae Takahashi, Yoshiro Ogoma, Masahiro Hatano

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23 Citations (Scopus)

Abstract

Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalFEBS Letters
Volume207
Issue number2
DOIs
Publication statusPublished - 1986 Oct 27

Keywords

  • (Yeast)
  • Cytochrome P-450
  • Expression
  • cDNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Shimizu, T., Sogawa, K., Fujii-Kuriyama, Y., Takahashi, M., Ogoma, Y., & Hatano, M. (1986). Expression of cytochrome P-450d by Saccharomyces cerevisiae. FEBS Letters, 207(2), 217-221. https://doi.org/10.1016/0014-5793(86)81491-0