Abstract
Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.
Original language | English |
---|---|
Pages (from-to) | 217-221 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 207 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1986 Oct 27 |
Keywords
- (Yeast)
- Cytochrome P-450
- Expression
- cDNA
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology