Expression of cytochrome P-450d by Saccharomyces cerevisiae

Toru Shimizu; Kazuhiro Sogawa; Yoshiaki Fujii-Kuriyama; Masae Takahashi; Yoshiro Ogoma; Masahiro Hatano

doi:10.1016/0014-5793(86)81491-0

Expression of cytochrome P-450_d by Saccharomyces cerevisiae

Toru Shimizu, Kazuhiro Sogawa, Yoshiaki Fujii-Kuriyama, Masae Takahashi, Yoshiro Ogoma, Masahiro Hatano

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article

23 Citations (Scopus)

Abstract

Rat liver microsomal cytochrome P-450_d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450_d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450_d was immunologically crossed with rat liver P-450_d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450_d, which is comparable to that reported previously for rat liver P-450_d. The expressed P-450_d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.

Original language	English
Pages (from-to)	217-221
Number of pages	5
Journal	FEBS Letters
Volume	207
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)81491-0
Publication status	Published - 1986 Oct 27

Keywords

(Yeast)
Cytochrome P-450
Expression
cDNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(86)81491-0

Fingerprint Dive into the research topics of 'Expression of cytochrome P-450<sub>d</sub> by Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this

Shimizu, T., Sogawa, K., Fujii-Kuriyama, Y., Takahashi, M., Ogoma, Y., & Hatano, M. (1986). Expression of cytochrome P-450_d by Saccharomyces cerevisiae. FEBS Letters, 207(2), 217-221. https://doi.org/10.1016/0014-5793(86)81491-0

@article{eb4978cf3f80405f81f2f70248b634ee,

title = "Expression of cytochrome P-450d by Saccharomyces cerevisiae",

abstract = "Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.",

keywords = "(Yeast), Cytochrome P-450, Expression, cDNA",

author = "Toru Shimizu and Kazuhiro Sogawa and Yoshiaki Fujii-Kuriyama and Masae Takahashi and Yoshiro Ogoma and Masahiro Hatano",

year = "1986",

month = oct,

day = "27",

doi = "10.1016/0014-5793(86)81491-0",

language = "English",

volume = "207",

pages = "217--221",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Expression of cytochrome P-450d by Saccharomyces cerevisiae

AU - Shimizu, Toru

AU - Sogawa, Kazuhiro

AU - Fujii-Kuriyama, Yoshiaki

AU - Takahashi, Masae

AU - Ogoma, Yoshiro

AU - Hatano, Masahiro

PY - 1986/10/27

Y1 - 1986/10/27

N2 - Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.

AB - Rat liver microsomal cytochrome P-450d was abundantly expressed in the yeast Saccharomyces cerevisiae by using a yeast-Escherichia coli shuttle vector consisting of rat liver P-450d cDNA and yeast acid phosphatase promoter. The expressed cytochrome P-450d was immunologically crossed with rat liver P-450d. The hydroxylase activity of estra-1,3,5(10)-triene-3,17β-diol was 11 nmol/min per nmol P-450d, which is comparable to that reported previously for rat liver P-450d. The expressed P-450d content was nearly 1% of total yeast protein as estimated from immunoblotting, hydroxylase activity and optical absorption of the reduced CO form.

KW - (Yeast)

KW - Cytochrome P-450

KW - Expression

KW - cDNA

UR - http://www.scopus.com/inward/record.url?scp=0023025187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023025187&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(86)81491-0

DO - 10.1016/0014-5793(86)81491-0

M3 - Article

C2 - 3533629

AN - SCOPUS:0023025187

VL - 207

SP - 217

EP - 221

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -