Expression of cyclooxygenase 2 by prostaglandin E2 in human endometrial adenocarcinoma cell line HEC-1B

I. Munir, K. Fukunaga, H. Kanasakifi, K. Miyazaki, T. Ohba, H. Okamura, E. Miyamoto

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The regulation of expression of cyclooxygenase 2 (COX-2) was investigated by treatment with PGE2 in human endometrial adenocarcinoma cell line HEC-1B. One μM PGE2 could stimulate the expression of COX-2 approximately twofold in this cell line. The same concentration of PGE2 also stimulated activation of mitogen-activated protein kinase (MAP kinase) and protein kinase B (PKB). PGE2-induced MAP kinase activation was sensitive to a MAP kinase kinase (MEK) inhibitor, PD098059, and a protein kinase A inhibitor, H-89. PD098059 and H-89 also partially inhibited the expression of COX-2 stimulated by PGE2. PGE2 could stimulate the activation of PKB, which was sensitive to phosphatidylinositol-3-OH kinase (PI3K) inhibitor, wortman. nin. Whereas wortmannin alone partially inhibited the expression of COX-2, a combination of wortmannin and PD098059 totally inhibited PGE2-mediated COX-2 expression. These results suggest that MAP kinase and PI3K pathways are stimulated with PGE2, and that both of these pathways are involved in the expression of COX-2. In addition, they also suggest that protein kinase A remains upstream of PGE2-induced activation of MAP kinase in HEC-1B cells.

Original languageEnglish
Pages (from-to)933-941
Number of pages9
JournalBiology of Reproduction
Volume63
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Kinases
  • Signal transducers
  • Signal transduction
  • Uterus

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of cyclooxygenase 2 by prostaglandin E<sub>2</sub> in human endometrial adenocarcinoma cell line HEC-1B'. Together they form a unique fingerprint.

Cite this