Expression of cloned calf prochymosin gene sequence in Escherichia coli

Nishimori Katsuhiko, Kawaguchi Yoshiyuki, Hidaka Makoto, Uozumi Takeshi, Beppu Teruhiko

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

An expression plasmid for calf prochymosin (prorennin) cDNA was constructed. The plasmid (pCR301) contains the lacUV5 promoter in front of the fused gene in which the codons for the N-terminal four amino acids of prochymosin cDNA were replaced with those for the N-terminal ten amino acids of β-galactosidase. Synthesis of the fused protein with the expected Mr was detected immunologically in Escherichia coli harboring pCR301. The product seemed to be localized in the cell membrane of the bacterial host.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalGene
Volume19
Issue number3
DOIs
Publication statusPublished - 1982 Oct

Keywords

  • Recombinant DNA
  • lac control
  • radioimmunodetection by western blotting

ASJC Scopus subject areas

  • Genetics

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