TY - JOUR
T1 - Expression of cloned calf prochymosin gene sequence in Escherichia coli
AU - Katsuhiko, Nishimori
AU - Yoshiyuki, Kawaguchi
AU - Makoto, Hidaka
AU - Takeshi, Uozumi
AU - Teruhiko, Beppu
PY - 1982/10
Y1 - 1982/10
N2 - An expression plasmid for calf prochymosin (prorennin) cDNA was constructed. The plasmid (pCR301) contains the lacUV5 promoter in front of the fused gene in which the codons for the N-terminal four amino acids of prochymosin cDNA were replaced with those for the N-terminal ten amino acids of β-galactosidase. Synthesis of the fused protein with the expected Mr was detected immunologically in Escherichia coli harboring pCR301. The product seemed to be localized in the cell membrane of the bacterial host.
AB - An expression plasmid for calf prochymosin (prorennin) cDNA was constructed. The plasmid (pCR301) contains the lacUV5 promoter in front of the fused gene in which the codons for the N-terminal four amino acids of prochymosin cDNA were replaced with those for the N-terminal ten amino acids of β-galactosidase. Synthesis of the fused protein with the expected Mr was detected immunologically in Escherichia coli harboring pCR301. The product seemed to be localized in the cell membrane of the bacterial host.
KW - Recombinant DNA
KW - lac control
KW - radioimmunodetection by western blotting
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U2 - 10.1016/0378-1119(82)90024-5
DO - 10.1016/0378-1119(82)90024-5
M3 - Article
C2 - 6295885
AN - SCOPUS:0020395569
VL - 19
SP - 337
EP - 344
JO - Gene
JF - Gene
SN - 0378-1119
IS - 3
ER -