TY - JOUR
T1 - Expression of CCR7 and its ligands CCL19/CCL21 in muscles of polymyositis
AU - Tateyama, Maki
AU - Fujihara, Kazuo
AU - Misu, Tatsuro
AU - Feng, Juan
AU - Onodera, Yoshiaki
AU - Itoyama, Yasuto
N1 - Funding Information:
This work was supported in part by the grants-in-aid for General Scientific Research of the Ministry of Education, Culture, Sports, Science and Technology of Japan (15390271, 15590875) and by Miyagi Medical Women's Association. The authors thank Mr. Brent Bell for reading the manuscript.
PY - 2006/11/15
Y1 - 2006/11/15
N2 - Polymyositis is an autoimmune disorder in which autoaggressive CD8+ T cells are important in the pathogenesis. However, the mechanisms underlying sustained recruitment of these cells in the muscle tissue are still unknown. CCR7 and its ligands CCL19 and CCL21 are a chemokine system related to mononuclear cell migration and antigen presentation, and are suggested to play a key role in several autoimmune disorders. We investigated the expression of CCR7, CCL19 and CCL21 in frozen muscles of polymyositis. In immunohistochemistry, CCR7 was expressed mainly on mononuclear cells that infiltrated in the endomysium of polymyositis. 34.8 ± 9.4% of endomysial mononuclear cells expressed CCR7. By double immunostaining, about 60% of endomysial CD8+ T cells that surrounded the nonnecrotic muscle fibers coexpressed CCR7. Because most endomysial CD8+ T cells expressed CD45RO, these were regarded as CD45RO+CCR7+CD8+ T cells. On the other hand, CCL19 was expressed mainly on muscle fibers in proximity to CCR7+ mononuclear cells, on the endothelium of the vessels and some mononuclear cells. CCL21 immunoreactivities were found on small numbers of mononuclear cells. In some cases, CCL21 immunoreactivities were also found on muscle fibers and the endothelium of vessels. In RT-PCR analysis, transcripts of CCR7 and CCL21 were detected in all the polymyositis muscles examined and that of CCL19 was detected in five out of seven polymyositis muscles. The CCL19,CCL21/CCR7 chemokine system is expressed in inflamed muscles of polymyositis and may be involved in the pathomechanism of polymyositis.
AB - Polymyositis is an autoimmune disorder in which autoaggressive CD8+ T cells are important in the pathogenesis. However, the mechanisms underlying sustained recruitment of these cells in the muscle tissue are still unknown. CCR7 and its ligands CCL19 and CCL21 are a chemokine system related to mononuclear cell migration and antigen presentation, and are suggested to play a key role in several autoimmune disorders. We investigated the expression of CCR7, CCL19 and CCL21 in frozen muscles of polymyositis. In immunohistochemistry, CCR7 was expressed mainly on mononuclear cells that infiltrated in the endomysium of polymyositis. 34.8 ± 9.4% of endomysial mononuclear cells expressed CCR7. By double immunostaining, about 60% of endomysial CD8+ T cells that surrounded the nonnecrotic muscle fibers coexpressed CCR7. Because most endomysial CD8+ T cells expressed CD45RO, these were regarded as CD45RO+CCR7+CD8+ T cells. On the other hand, CCL19 was expressed mainly on muscle fibers in proximity to CCR7+ mononuclear cells, on the endothelium of the vessels and some mononuclear cells. CCL21 immunoreactivities were found on small numbers of mononuclear cells. In some cases, CCL21 immunoreactivities were also found on muscle fibers and the endothelium of vessels. In RT-PCR analysis, transcripts of CCR7 and CCL21 were detected in all the polymyositis muscles examined and that of CCL19 was detected in five out of seven polymyositis muscles. The CCL19,CCL21/CCR7 chemokine system is expressed in inflamed muscles of polymyositis and may be involved in the pathomechanism of polymyositis.
KW - CCL19
KW - CCR7
KW - Immunohistochemistry
KW - Polymyositis
KW - RT-PCR
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U2 - 10.1016/j.jns.2006.06.021
DO - 10.1016/j.jns.2006.06.021
M3 - Article
C2 - 16887149
AN - SCOPUS:33750504632
VL - 249
SP - 158
EP - 165
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 2
ER -