Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor

Osamu Higuchi, Kensaku Mizuno, George F. Vande Woude, Toshikazu Nakamura

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a Kd value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.

Original languageEnglish
Pages (from-to)282-286
Number of pages5
JournalFEBS Letters
Volume301
Issue number3
DOIs
Publication statusPublished - 1992 Apr 27
Externally publishedYes

Keywords

  • HGF
  • HGF receptor
  • Hepatocyte growth factor
  • Mitogenic signal
  • c-met Proto-oncogene

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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