Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor

Osamu Higuchi; Kensaku Mizuno; George F. Vande Woude; Toshikazu Nakamura

doi:10.1016/0014-5793(92)80257-H

Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor

Osamu Higuchi, Kensaku Mizuno, George F. Vande Woude, Toshikazu Nakamura

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a K_d value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.

Original language	English
Pages (from-to)	282-286
Number of pages	5
Journal	FEBS Letters
Volume	301
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(92)80257-H
Publication status	Published - 1992 Apr 27
Externally published	Yes

Keywords

HGF
HGF receptor
Hepatocyte growth factor
Mitogenic signal
c-met Proto-oncogene

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(92)80257-H

Cite this

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title = "Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor",

abstract = "By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a Kd value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.",

keywords = "HGF, HGF receptor, Hepatocyte growth factor, Mitogenic signal, c-met Proto-oncogene",

author = "Osamu Higuchi and Kensaku Mizuno and {Vande Woude}, {George F.} and Toshikazu Nakamura",

year = "1992",

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doi = "10.1016/0014-5793(92)80257-H",

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TY - JOUR

T1 - Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor

AU - Higuchi, Osamu

AU - Mizuno, Kensaku

AU - Vande Woude, George F.

AU - Nakamura, Toshikazu

PY - 1992/4/27

Y1 - 1992/4/27

N2 - By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a Kd value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.

AB - By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a Kd value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.

KW - HGF

KW - HGF receptor

KW - Hepatocyte growth factor

KW - Mitogenic signal

KW - c-met Proto-oncogene

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Expression of c-met proto-oncogene in COS cells induces the signal transducing high-affinity receptor for hepatocyte growth factor

Abstract

Keywords

ASJC Scopus subject areas

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