Expression of AODEF, a B-functional MADS-box gene, in stamens and inner tepals of the dioecious species Asparagus officinalis L.

J. H. Park, Y. Ishikawa, R. Yoshida, A. Kanno, T. Kameya

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)

Abstract

Garden asparagus (Asparagus officinalis L.) is a dioecious species with male and female flowers on separate unisexual individuals. Since B- and C-functional MADS-box genes specify male and female reproductive organs, it is important to characterize these genes to clarify the mechanism of sex determination in monoecious and dioecious species. In this study, we isolated and characterized AODEF 1, a B-functional gene in the development of male and female flowers of A. officinalis. Southern hybridization identified a single copy of AODEF gene in asparagus genome. Northern blot analysis showed that this gene was specifically expressed in flower buds and not in vegetative tissues. In situ hybridization showed that during early hermaphrodite stages, AODEF gene was expressed in the inner tepal and stamen whorls (whorls 2 and 3, respectively), but not in the outer tepals (whorl 1), in both male and female flowers. In late unisexual developmental stages, the expression of AODEF gene was still detected in the inner tepals and stamens of male flowers, but the expression was reduced in whorls 2 and 3 of female flowers. Our results suggest that AODEF gene is probably not involved in tepal development in asparagus and that the expression of AODEF gene is probably controlled directly or indirectly by sex determination gene in the late developmental stages.

Original languageEnglish
Pages (from-to)867-875
Number of pages9
JournalPlant Molecular Biology
Volume51
Issue number6
DOIs
Publication statusPublished - 2003 Apr

Keywords

  • Asparagus officinalis L.
  • DEFICIENS
  • Dioecious
  • Flower development
  • MADS-box gene

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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