EXPRESSION OF AN Anacystis nidulans PHOTOLYASE GENE IN Escherichia coli; FUNCTIONAL COMPLEMENTATION AND MODIFIED ACTION SPECTRUM OF PHOTOREACTIVATION

Masashi Takao, Atsushi Oikawa, Andre P.M. Eker, Akira Yasui

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Abstract— The Anacystis nidulans photolyase gene inserted in an expression vector plasmid was introduced into Escherichia coli cells and the production of Anacystis photolyase protein was confirmed by reaction with antibodies raised against photolyase purified from A. nidulans cells. The Anacystis photolyase functioned in photoreactivation repair defective E. coli cells. The E. coli transformants exhibited an action spectrum with a maximum around 380 nm similar to that of E. coli photolyase in contrast with the action spectrum of A. nidulans cells which has a maximum at 437 nm. These results indicate that the Anacystis photolyase produced in E. coli cells has enzymatic activity in spite of the apparent lack of its intrinsic 8‐hydroxy‐5‐deazaflavin cofactor.

Original languageEnglish
Pages (from-to)633-637
Number of pages5
JournalPhotochemistry and Photobiology
Volume50
Issue number5
DOIs
Publication statusPublished - 1989 Nov

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of 'EXPRESSION OF AN Anacystis nidulans PHOTOLYASE GENE IN Escherichia coli; FUNCTIONAL COMPLEMENTATION AND MODIFIED ACTION SPECTRUM OF PHOTOREACTIVATION'. Together they form a unique fingerprint.

  • Cite this