Expression of adrenomedullin2/intermedin in human brain, heart, and kidney

Ryo Morimoto, Fumitoshi Satoh, Osamu Murakami, Kazuhito Totsune, Takashi Suzuki, Hironobu Sasano, Sadayoshi Ito, Kazuhiro Takahashi

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Adrenomedullin2/intermedin (AM2/IMD) is a novel member of the calcitonin/calcitonin gene-related peptide (CGRP) family. In the present study, we developed a specific radioimmunoassay of human AM2/IMD. Expression of AM2/IMD was studied in the human brain, pituitary, heart and kidney obtained at autopsy by radioimmunoassay and immunocytochemistry. Immunoreactive-AM2/IMD was detected by radioimmunoassay in human brains (range; 0.163-1.495 pmol/g wet weight), pituitaries (4.46 ± 0.689 pmol/g wet weight, mean ± S.E.M, n = 3), left ventricles of hearts (0.251 ± 0.0321 pmol/g wet weight, n = 4), kidneys (3.49 ± 1.18 pmol/g wet weight, n = 5), and plasma obtained at healthy subjects (24.7 ± 1.78 pmol/l, n = 3). Reverse-phase high performance liquid chromatography showed that immunoreactive-AM2/IMD in human brain, kidney and plasma extracts were eluted in the position of authentic AM2/IMD. Additional peaks eluted earlier were found in the brain tissue and plasma. Immunocytochemistry showed that immunoreactive-AM2/IMD was localized in paraventricular and supraoptic nuclei of hypothalamus, anterior and posterior lobes of pituitary, cardiomyocytes, pericardial adipocytes, vascular endothelial cells of pericardial veins, and vascular smooth muscle cells of coronary arteries and renal arterioles as well as in renal tubular cells. The present study has shown expression of AM2/IMD in various types of cells in the central nervous system and the cardiovascular system, and suggested possible (patho)physiological roles of AM2/IMD in these systems.

Original languageEnglish
Pages (from-to)1095-1103
Number of pages9
JournalPeptides
Volume28
Issue number5
DOIs
Publication statusPublished - 2007 May

Keywords

  • Adrenomedullin
  • Heart
  • Intermedin
  • Kidney
  • Pituitary
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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